Drosophila semaphorin2b is required for the axon guidance of a subset of embryonic neurons
Article first published online: 30 MAY 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 242, Issue 7, pages 861–873, July 2013
How to Cite
Emerson, M. M., Long, J. B. and Van Vactor, D. (2013), Drosophila semaphorin2b is required for the axon guidance of a subset of embryonic neurons. Dev. Dyn., 242: 861–873. doi: 10.1002/dvdy.23979
- Issue published online: 17 JUN 2013
- Article first published online: 30 MAY 2013
- Accepted manuscript online: 20 APR 2013 03:13AM EST
- Manuscript Accepted: 21 MAR 2013
- Manuscript Revised: 20 MAR 2013
- Manuscript Received: 11 NOV 2012
- NIH/NINDS. Grant Number: NS35909
- axon guidance;
Background: The process of axon guidance is important in establishing functional neural circuits. The differential expression of cell-autonomous axon guidance factors is crucial for allowing axons of different neurons to take unique trajectories in response to spatially and temporally restricted cell non-autonomous axon guidance factors. A key motivation in the field is to provide adequate explanations for axon behavior with respect to the differential expression of these factors. Results: We report the characterization of a predicted secreted semaphorin family member, semaphorin2b (Sema-2b) in Drosophila embryonic axon guidance. Misexpression of Sema-2b in neurons causes highly penetrant axon guidance phenotypes in specific longitudinal and motoneuron pathways; however, expression of Sema-2b in muscles traversed by these motoneurons has no effect on axon guidance. In Sema-2b loss-of-function embryos, specific motoneuron and interneuron axon pathways display guidance defects. Specific visualization of the neurons that normally express Sema-2b reveals that this neuronal cohort is strongly affected by Sema-2b loss-of-function alleles. Conclusions: While secreted semaphorins have been implicated as cell non-autonomous chemorepellants in a variety of contexts, here we report previously undescribed Sema-2b loss-of-function and misexpression phenotypes that are consistent with a cell-autonomous role for Sema-2b. Developmental Dynamics 242:861–873, 2013. © 2013 Wiley Periodicals, Inc.