RNA interference by feeding in vitro–synthesized double-stranded RNA to planarians: Methodology and dynamics



COVER PHOTOGRAPH: Low magnification image of planarian flatworms (Schmidtea mediterranea) fed with liver paste containing either red, green, yellow, or blue food dye. Uptake of colored liver paste containing in vitro synthesized double-stranded RNA is readily visualized in the planarian gut. Ingestion and processing of double-stranded RNA in the planarian gut leads to systemic RNA-interference. Image credit: Labib Rouhana and Phillip A. Newmark, Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign. From RNA Interference by feeding in vitro-synthesized double-stranded RNA to planarians, Labib Rouhana, Jennifer A. Weiss, David J. Forsthoefel, Hayoung Lee, Ryan S. King, Takeshi Inoue, Norito Shibata, Kiyokazu Agata, and Phillip A. Newmark, Developmental Dynamics 242:718–730, 2013.