Drs. Brand-Saberi and Theiss contributed equally to this work.
Combination of in ovo electroporation and time-lapse imaging to study migrational events in chicken embryos
Article first published online: 24 FEB 2014
Copyright © 2013 Wiley Periodicals, Inc.
Volume 243, Issue 5, pages 690–698, May 2014
How to Cite
Masyuk, M., Morosan-Puopolo, G., Brand-Saberi, B. and Theiss, C. (2014), Combination of in ovo electroporation and time-lapse imaging to study migrational events in chicken embryos. Dev. Dyn., 243: 690–698. doi: 10.1002/dvdy.24109
- Issue published online: 17 APR 2014
- Article first published online: 24 FEB 2014
- Accepted manuscript online: 27 DEC 2013 10:34PM EST
- Manuscript Accepted: 21 DEC 2013
- Manuscript Revised: 16 DEC 2013
- Manuscript Received: 30 JUL 2013
- confocal laser scanning microscopy;
- spinning disc confocal microscopy;
- tissue slice cultures;
- Rose chamber
Background: During embryonic development cell migration plays a principal role in several processes. In past decades, many studies were performed to investigate migrational events, occurring during embryonic organogenesis, neurogenesis, gliogenesis or myogenesis, just to name a few. Although different common techniques are already used for this purpose, one of their major limitations is the static character. However, cell migration is a sophisticated and highly dynamic process, wherefore new appropriate technologies are required to investigate this event in all its complexity. Results and Conclusions: Here we report a novel approach for dynamic analysis of cell migration within embryonic tissue. We combine the modern transfection method of in ovo electroporation with the use of tissue slice culture and state-of-the-art imaging techniques, such as confocal laser scanning microscopy or spinning disc confocal microscopy, and thus, develop a method to study live the migration of myogenic precursors in chicken embryos. The conditions and parameters used in this study allow long-term imaging for up to 24 hr. Our protocol can be easily adapted for investigations of a variety of other migrational events and provides a novel conception for dynamic analysis of migration during embryonic development. Developmental Dynamics 243:690–698, 2014. © 2013 Wiley Periodicals, Inc.