Ann Easton, Lydia Li, Ouma Onguka, Joseph S. Ramahi, Rowena Suriben, Linda A. Szabo, Damilla Teng, and Baouyen Tran contributed equally to the work.
Patterns & Phenotypes
Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo
Article first published online: 1 APR 2014
© 2014 Wiley Periodicals, Inc.
Volume 243, Issue 6, pages 833–843, June 2014
How to Cite
Galli, L. M., Munji, R. N., Chapman, S. C., Easton, A., Li, L., Onguka, O., Ramahi, J. S., Suriben, R., Szabo, L. A., Teng, C., Tran, B., Hannoush, R. N. and Burrus, L. W. (2014), Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo. Dev. Dyn., 243: 833–843. doi: 10.1002/dvdy.24123
- Issue published online: 22 MAY 2014
- Article first published online: 1 APR 2014
- Accepted manuscript online: 6 MAR 2014 02:21AM EST
- Manuscript Accepted: 4 MAR 2014
- Manuscript Revised: 3 MAR 2014
- Manuscript Received: 1 OCT 2013
- National Science Foundation. Grant Numbers: RUI MCB-1244602, RUI IOS-0950892
- National Institutes of Health. Grant Numbers: 1R15HD070206-01A1, 2 SO6 GM52588, 1R15HD070206-01A1, R01DC009236
Background: WNT1 and WNT3A drive a dorsal to ventral gradient of β-catenin-dependent Wnt signaling in the developing spinal cord. However, the identity of the receptors mediating downstream functions remains poorly understood. Results: In this report, we show that the spatiotemporal expression patterns of FZD10 and WNT1/WNT3A are highly correlated. We further show that in the presence of LRP6, FZD10 promotes WNT1 and WNT3A signaling using an 8xSuperTopFlash reporter assay. Consistent with a functional role for FZD10, we demonstrate that FZD10 is required for proliferation in the spinal cord. Finally, by using an in situ proximity ligation assay, we observe an interaction between FZD10 and WNT1 and WNT3A proteins. Conclusions: Together, our results identify FZD10 as a receptor for WNT1 and WNT3A in the developing chick spinal cord. Developmental Dynamics 243:833–843, 2014. © 2014 Wiley Periodicals, Inc.