R-spondins/Lgrs expression in tooth development

Authors

  • Maiko kawasaki,

    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
    2. Division of Bio-Prosthodontics, Department of Oral Health Science, Course for Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
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    • Drs. Kawasaki and Porntaveetus contributed equally to this work.

  • Thantrira Porntaveetus,

    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
    2. Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
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    • Drs. Kawasaki and Porntaveetus contributed equally to this work.

  • Katsushige Kawasaki,

    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
    2. Department of Pedatric Dentistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
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  • Shelly Oommen,

    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
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  • Yoko Otsuka-Tanaka,

    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
    2. Department of Special Needs Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
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  • Mitsue Hishinuma,

    1. Department of Special Needs Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
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  • Takato Nomoto,

    1. Department of Special Needs Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Japan
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  • Takeyasu Maeda,

    1. Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
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  • Keiyo Takubo,

    1. Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University School of Medicine, Tokyo, Japan
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  • Toshio Suda,

    1. Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University School of Medicine, Tokyo, Japan
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  • Paul T. Sharpe,

    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
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  • Atsushi Ohazama

    Corresponding author
    1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom
    2. Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
    • Correspondence to: Atsushi Ohazama, Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, 2–5274, Gakkocho-dori, Chuo-ku, Niigata 951–8514, Japan. E-mail: atsushiohazama@dent.niigata-u.ac.jp

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Abstract

Background: Tooth development is highly regulated in mammals and it is regulated by networks of signaling pathways (e. g. Tnf, Wnt, Shh, Fgf and Bmp) whose activities are controlled by the balance between ligands, activators, inhibitors and receptors. The members of the R-spondin family are known as activators of Wnt signaling, and Lgr4, Lgr5, and Lgr6 have been identified as receptors for R-spondins. The role of R-spondin/Lgr signaling in tooth development, however, remains unclear. Results: We first carried out comparative in situ hybridization analysis of R-spondins and Lgrs, and identified their dynamic spatio-temporal expression in murine odontogenesis. R-spondin2 expression was found both in tooth germs and the tooth-less region, the diastema. We further examined tooth development in R-spondin2 mutant mice, and although molars and incisors exhibited no significant abnormalities, supernumerary teeth were observed in the diastema. Conclusions: R-spondin/Lgr signaling is thus involved in tooth development. Developmental Dynamics 243:844–851, 2014. © 2014 Wiley Periodicals, Inc.

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