Identification of E2F target genes that are rate limiting for dE2F1-dependent cell proliferation (pages 1695–1707)
Anabel Herr, Michelle Longworth, Jun-Yuan Ji, Michael Korenjak, David M. MacAlpine and Nicholas J. Dyson
Version of Record online: 17 SEP 2012 | DOI: 10.1002/dvdy.23857
We have used a list of E2F-regulated genes that has been generated by microarray analysis for a genetic approach to ask which of these targets are important for E2F function in vivo.
Our results show that more than half of the genes tested are able to dominantly modify dE2F1-dependent phenotypes, indicating that the expression levels of most dE2F1-regulated genes are rate-limiting for dE2F1 function.
We show that the depletion of Orc5 elevates the expression of a dE2F1-regulated gene.
Our results reveal the existence of an important feedback mechanism that can elevate dE2F1-dependent transcription in cells where ORC levels are low.