This article is a US Government work and, as such, is in the public domain in the United States of America.
Targeted gene expression in the zebrafish prechordal plate†
Article first published online: 28 NOV 2006
Published 2006 Wiley-Liss, Inc.
Volume 44, Issue 12, pages 584–588, December 2006
How to Cite
Inbal, A., Topczewski, J. and Solnica-Krezel, L. (2006), Targeted gene expression in the zebrafish prechordal plate. Genesis, 44: 584–588. doi: 10.1002/dvg.20253
- Issue published online: 5 DEC 2006
- Article first published online: 28 NOV 2006
- Manuscript Received: 26 AUG 2006
- Manuscript Accepted: 26 AUG 2006
- National Institutes of Health (NIH)
- Vanderbilt Zebrafish Initiative AVCF
Targeted gene expression is a powerful tool for understanding gene function in vivo. In zebrafish, overexpression of gene products is typically accomplished ubiquitously, without temporal and spatial specificity. However, the yeast Gal4/UAS system can be used for targeted gene expression in zebrafish. Here we describe the generation and characterization of Tg[gsc: Gal4-VP16] transgenic zebrafish lines that harbor a construct encoding Gal4-VP16 transcriptional activator under the control of a fragment of the goosecoid gene promoter. Tg[gsc:Gal4-VP16] embryos express Gal4-VP16 RNA in presumptive prechordal plate mesendoderm during late blastula and throughout gastrulation. By crossing these fish to Tg[UAS-GFP] transgenic fish, we show that the gsc:Gal4-VP16 transgene is capable of driving strong expression of a target gene in the prechordal plate and its derivatives during gastrulation and segmentation. Thus, the use of Tg[gsc:Gal4-VP16] fish can help in understanding gene function in the prechordal plate, an embryonic structure that is crucial for normal neural patterning. genesis 44:584–588, 2006. Published 2006 Wiley-Liss, Inc.