High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis
Article first published online: 26 APR 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 45, Issue 5, pages 307–317, May 2007
How to Cite
Awazu, S., Matsuoka, T., Inaba, K., Satoh, N. and Sasakura, Y. (2007), High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis. Genesis, 45: 307–317. doi: 10.1002/dvg.20290
- Issue published online: 1 MAY 2007
- Article first published online: 26 APR 2007
- Manuscript Accepted: 5 MAR 2007
- Manuscript Revised: 29 JAN 2007
- Manuscript Received: 5 OCT 2006
- Ministry of Education, Culture, Sports, Science and Technology, Japan. Grant Numbers: 1600873, 1702260, 17018018, 18770193, 17207013
- CREST, Japan Science and Technology Agency, Japan
The enhancer trap approach utilizing transposons yields us information about gene functions and gene expression patterns. In the ascidian Ciona intestinalis, transposon-based transgenesis and insertional mutagenesis were achieved with a Tc1/mariner transposon Minos. We report development of a novel technique for enhancer trap in C. intestinalis. This technique uses remobilization of Minos in the Ciona genome. A Minos vector for enhancer trap was constructed and a tandem array insertion of the vector was introduced into the Ciona genome to create a mutator line. Minos was remobilized in Ciona chromosomes to create new insertions by providing transposases. These transposase-introduced animals were crossed with wild-type animals. Nearly 80% of F1 families showed novel GFP expression patterns. This high-throughput enhancer trap screen will be useful to create new marker transgenic lines showing reporter gene expression in specific tissues and to identify novel patterns of gene expression. genesis 45:307–317, 2007. © 2007 Wiley-Liss, Inc.