Malgorzata E. Rula and Kathy Q. Cai contributed equally to this work.
Cell autonomous sorting and surface positioning in the formation of primitive endoderm in embryoid bodies†
Version of Record online: 15 MAY 2007
Published 2007 Wiley-Liss, Inc.
Volume 45, Issue 6, pages 327–338, June 2007
How to Cite
Rula, M. E., Cai, K. Q., Moore, R., Yang, D.-H., Staub, C. M., Capo-chichi, C. D., Jablonski, S. A., Howe, P. H., Smith, E. R. and Xu, X.-X. (2007), Cell autonomous sorting and surface positioning in the formation of primitive endoderm in embryoid bodies. Genesis, 45: 327–338. doi: 10.1002/dvg.20298
This article is a US Government work and, as such, is in the public domain in the United States of America.
- Issue online: 11 JUN 2007
- Version of Record online: 15 MAY 2007
- Manuscript Accepted: 16 MAR 2007
- Manuscript Received: 26 OCT 2006
- NIH. Grant Numbers: R01 CA099471, R01 CA79716, R01 CA75389
- Commonwealth of Pennsylvania
- primitive endoderm;
- surface positioning;
- Disabled-2 (Dab2);
- cell sorting;
- embryonic stem cells;
- F9 embryonal carcinoma cells
The differentiation and formation of the primitive endoderm in early embryos can be mimicked in vitro by the aggregation of embryonic stem cells to form embryoid bodies. We present morphological evidence that primitive endoderm cells often first locate in the interior of embryoid bodies and subsequently migrate to the surface. Cell mixing experiments indicate that surface positioning is an intrinsic property of endoderm epithelial cells. Moreover, Disabled-2 (Dab2) is required for surface sorting and positioning of the endoderm cells: when Dab2 expression was eliminated, the differentiated endoderm epithelial cells distributed throughout the interior of the embryoid bodies. Surprisingly, E-cadherin is dispensable for primitive endoderm differentiation and surface sorting in embryoid bodies. These results support the model that primitive endoderm cells first emerge in the interior of the inner cell mass and are subsequently sorted to the surface to form the primitive endoderm. genesis 45: 327–338, 2007. Published 2007 Wiley-Liss, Inc.