Cre reporter mouse expressing a nuclear localized fusion of GFP and β-galactosidase reveals new derivatives of Pax3-expressing precursors

Authors

  • Jason Z. Stoller,

    1. Division of Neonatology, Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia 19104
    2. Department of Cell and Developmental Biology and the Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia 19104
    Search for more papers by this author
  • Karl R. Degenhardt,

    1. Division of Cardiology, Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia 19104
    2. Department of Cell and Developmental Biology and the Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia 19104
    Search for more papers by this author
  • Li Huang,

    1. Division of Neonatology, Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia 19104
    2. Department of Cell and Developmental Biology and the Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia 19104
    Search for more papers by this author
  • Diane D. Zhou,

    1. Department of Cell and Developmental Biology and the Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia 19104
    Search for more papers by this author
  • Min Min Lu,

    1. Department of Cell and Developmental Biology and the Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia 19104
    Search for more papers by this author
  • Jonathan A. Epstein

    Corresponding author
    1. Department of Cell and Developmental Biology and the Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia 19104
    • 954 BRB II, 421 Curie Blvd., Philadelphia PA 19104
    Search for more papers by this author

Abstract

A new Cre-reporter strain of mouse has been developed that expresses a fusion protein derived from the lacZ gene fused to GFP with a nuclear localization signal. This construct is expressed from the ROSA26 locus upon Cre-mediated recombination that removes a loxP-flanked PGK-neo cassette, thus allowing for detection of Cre activity in all tissues. This reporter strain, which is similar to prior R26R and R26EGFP strains, has certain advantages related to the nuclear expression and the combined expression of both β-galactosidase and GFP activities. We show that the use of this newly developed reporter line allows for enhanced resolution, detection and co-localization. Thus, we report a previously unrecognized subset of venous endothelial cells derived from Pax3 expressing precursors. genesis 46:200–204, 2008. © 2008 Wiley-Liss, Inc.

Ancillary