The IRG mouse: A two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ

Authors

  • Rita De Gasperi,

    1. Research and Development, James J. Peters Department of Veterans Affairs Medical Center, Bronx, New York
    2. Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
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  • Anne B. Rocher,

    1. Department of Neuroscience, Mount Sinai School of Medicine, New York, New York
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  • Miguel A. Gama Sosa,

    1. Research and Development, James J. Peters Department of Veterans Affairs Medical Center, Bronx, New York
    2. Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
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  • Susan L. Wearne,

    1. Department of Neuroscience, Mount Sinai School of Medicine, New York, New York
    2. Center for Biomathematical Sciences, Mount Sinai School of Medicine, New York, New York
    3. Computational Neurobiology and Imaging Center, Mount Sinai School of Medicine, New York, New York
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  • Gissel M. Perez,

    1. Research and Development, James J. Peters Department of Veterans Affairs Medical Center, Bronx, New York
    2. Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
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  • Victor L. Friedrich Jr.,

    1. Department of Neuroscience, Mount Sinai School of Medicine, New York, New York
    2. Microscopy Shared Research Facility, Mount Sinai School of Medicine, New York, New York
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  • Patrick R. Hof,

    1. Department of Neuroscience, Mount Sinai School of Medicine, New York, New York
    2. Computational Neurobiology and Imaging Center, Mount Sinai School of Medicine, New York, New York
    3. Department of Geriatrics and Adult Development, Mount Sinai School of Medicine, New York, New York
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  • Gregory A. Elder

    Corresponding author
    1. Research and Development, James J. Peters Department of Veterans Affairs Medical Center, Bronx, New York
    2. Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
    3. Rehabilitation Medicine Service, James J. Peters Department of Veterans Affairs Medical Center, Bronx, New York
    • James J. Peters VA Medical Center, Research and Development (3F22), 130 West Kingsbridge Road, Bronx, NY 10468, NY
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  • This article is a US Government work and, as such, is in the public domain in the United States of America.

Abstract

The Cre-loxP system is widely used for making conditional alterations to the mouse genome. Cre-mediated recombination is frequently monitored using reporter lines in which Cre expression activates a reporter gene driven by a ubiquitous promoter. Given the distinct advantages of fluorescent reporters, we developed a transgenic reporter line, termed IRG, in which DsRed-Express, a red fluorescent protein (RFP) is expressed ubiquitously prior to Cre-mediated recombination and an enhanced green fluorescent protein (EGFP) following recombination. Besides their utility for monitoring Cre-mediated recombination, we show that in IRG mice red and green native fluorescence can be imaged simultaneously in thick tissue sections by confocal microscopy allowing for complex reconstructions to be created that are suitable for analysis of neuronal morphologies as well as neurovascular interactions in brain. IRG mice should provide a versatile tool for analyzing complex cellular relationships in both neural and nonneural tissues. genesis 46:308–317, 2008. Published 2008 Wiley-Liss, Inc.

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