Terence D. Capellini and Matthew P. Dunn contributed equally to this work.
Conservation of notochord gene expression across chordates: Insights from the Leprecan gene family
Article first published online: 17 SEP 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Chordate Origins and Evolution
Volume 46, Issue 11, pages 683–696, November 2008
How to Cite
Capellini, T. D., Dunn, M. P., Passamaneck, Y. J., Selleri, L. and Di Gregorio, A. (2008), Conservation of notochord gene expression across chordates: Insights from the Leprecan gene family. Genesis, 46: 683–696. doi: 10.1002/dvg.20406
- Issue published online: 10 NOV 2008
- Article first published online: 17 SEP 2008
- Manuscript Accepted: 20 MAY 2008
- Manuscript Revised: 28 MAR 2008
- Manuscript Received: 3 JAN 2008
- NIH/NICHD. Grant Numbers: R01HD050704, R01HD43997
- March of Dimes Birth Defects Foundation (Basil O'Connor Starter Scholar Research Award). Grant Number: 5-FY03-153
- prolyl 3-hydroxylase
The notochord is a defining character of the chordates, and the T-box transcription factor Brachyury has been shown to be required for notochord development in all chordates examined. In the ascidian Ciona intestinalis, at least 44 notochord genes have been identified as bona fide transcriptional targets of Brachyury. We examined the embryonic expression of a subset of murine orthologs of Ciona Brachyury target genes in the notochord to assess its conservation throughout chordate evolution. We focused on analyzing the Leprecan gene family, which in mouse is composed of three genes, as opposed to the single-copy Ciona gene. We found that all three mouse Leprecan genes are expressed in the notochord. Additionally, while Leprecan expression in C. intestinalis is confined to the notochord, expression of its mouse orthologs includes dorsal root ganglia, limb buds, branchial arches, and developing kidneys. These results have interesting implications for the evolution and development of chordates. genesis 46:683–696, 2008. © 2008 Wiley-Liss, Inc.