Article

A new mouse model for temporal- and tissue-specific control of extracellular superoxide dismutase

  1. Yani Zou1,
  2. Chih-Hsin Chen1,
  3. John R. Fike2,
  4. Ting-Ting Huang1,3,*

Article first published online: 22 JAN 2009

DOI: 10.1002/dvg.20470

Issue

genesis

genesis

Volume 47, Issue 3, pages 142–154, March 2009

Additional Information

How to Cite

Zou, Y., Chen, C.-H., Fike, J. R. and Huang, T.-T. (2009), A new mouse model for temporal- and tissue-specific control of extracellular superoxide dismutase. Genesis, 47: 142–154. doi: 10.1002/dvg.20470

Author Information

  1. 1

    Department of Neurology and Neurological Sciences, Stanford University, Stanford, California

  2. 2

    Departments of Neurological Surgery and Radiation Oncology, University of California, San Francisco, California

  3. 3

    Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, California

*Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA and GRECC, VAPAHCS, CA

Publication History

  1. Issue published online: 23 MAR 2009
  2. Article first published online: 22 JAN 2009
  3. Manuscript Accepted: 13 OCT 2008
  4. Manuscript Revised: 15 SEP 2008
  5. Manuscript Received: 17 JUN 2008

Funded by

  • National Institutes of Health. Grant Numbers: AG24400, NS46051
  • Palo Alto Institute for Research and Education (PAIRE)
  • VA Palo Alto Health Care System

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Keywords:

  • EC-SOD;
  • TRE promoter;
  • CamKII-tTA;
  • LAP-tTA;
  • transgenic mouse model

Abstract

The extracellular isoform of superoxide dismutase (EC-SOD, Sod3) plays a protective role against various diseases and injuries mediated by oxidative stress. To investigate the pathophysiological roles of EC-SOD, we generated tetracycline-inducible Sod3 transgenic mice and directed the tissue-specific expression of transgenes by crossing Sod3 transgenic mice with tissue-specific transactivator transgenics. Double transgenic mice with liver-specific expression of Sod3 showed increased EC-SOD levels predominantly in the plasma as the circulating form, whereas double transgenic mice with neuronal-specific expression expressed higher levels of EC-SOD in hippocampus and cortex with intact EC-SOD as the dominant form. EC-SOD protein levels also correlated well with increased SOD activities in double transgenic mice. In addition to enabling tissue-specific expression, the transgene expression can be quickly turned on and off by doxycycline supplementation in the mouse chow. This mouse model, thus, provides the flexibility for on–off control of transgene expression in multiple target tissues. genesis 47:142–154, 2009. © 2009 Wiley-Liss, Inc.

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