Article

Pax6 regulation of Math5 during mouse retinal neurogenesis

  1. Amy N. Riesenberg1,
  2. Tien T. Le1,
  3. Minde I. Willardsen2,
  4. David C. Blackburn3,
  5. Monica L. Vetter2,
  6. Nadean L. Brown1,*

Article first published online: 10 FEB 2009

DOI: 10.1002/dvg.20479

Issue

genesis

genesis

Volume 47, Issue 3, pages 175–187, March 2009

Additional Information

How to Cite

Riesenberg, A. N., Le, T. T., Willardsen, M. I., Blackburn, D. C., Vetter, M. L. and Brown, N. L. (2009), Pax6 regulation of Math5 during mouse retinal neurogenesis. Genesis, 47: 175–187. doi: 10.1002/dvg.20479

Author Information

  1. 1

    Division of Developmental Biology, Children's Hospital Research Foundation and Departments of Pediatrics and Ophthalmology, University of Cincinnati School of Medicine, Cincinnati, Ohio

  2. 2

    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah

  3. 3

    Museum of Comparative Zoology and Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts

*Division of Developmental Biology, Children's Hospital Research Foundation and Departments of Pediatrics and Ophthalmology, University of Cincinnati School of Medicine, Cincinnati, OH 45229, United States

Publication History

  1. Issue published online: 23 MAR 2009
  2. Article first published online: 10 FEB 2009
  3. Manuscript Accepted: 26 OCT 2008
  4. Manuscript Revised: 14 OCT 2008
  5. Manuscript Received: 20 AUG 2008

Funded by

  • NIH. Grant Number: EY12274
  • NIH. Grant Number: EY13612

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Keywords:

  • retina;
  • neurogenesis;
  • Math5;
  • Pax6;
  • transcriptional regulation

Abstract

Activation of the bHLH factor Math5 (Atoh7) is an initiating event for mammalian retinal neurogenesis, as it is critically required for retinal ganglion cell formation. However, the cis-regulatory elements and trans-acting factors that control Math5 expression are largely unknown. Using a combination of transgenic mice and bioinformatics, we identified a phylogenetically conserved regulatory element that is required to activate Math5 transcription during early retinal neurogenesis. This element drives retinal expression in vivo, in a cross-species transgenic assay. Previously, Pax6 was shown to be necessary for the initiation of Math5 mRNA expression. We extend this finding by showing that the Math5 retinal enhancer also requires Pax6 for its activation, via Pax6 binding to a highly conserved binding site. In addition, our data reveal that other retinal factors are required for accurate regulation of Math5 by Pax6. genesis 47:175–187, 2009. © 2009 Wiley-Liss, Inc.

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