Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus

Authors

  • Benjamin Schlager,

    1. Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany
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    • Benjamin Schlager and Xiaoyue Wang contributed equally to this work.

  • Xiaoyue Wang,

    1. Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany
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    • Benjamin Schlager and Xiaoyue Wang contributed equally to this work.

  • Georg Braach,

    1. Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany
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  • Ralf J. Sommer

    Corresponding author
    1. Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany
    • Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, Spemannstrasse 37; D-72076 Tübingen, Germany
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Abstract

We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus. genesis 47:300–304, 2009. © 2009 Wiley-Liss, Inc.

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