Technology Report

Generation of red fluorescent protein transgenic dogs

  1. So Gun Hong1,
  2. Min Kyu Kim2,
  3. Goo Jang1,
  4. Hyun Ju Oh1,
  5. Jung Eun Park1,
  6. Jung Taek Kang1,
  7. Ok Jae Koo1,
  8. Teoan Kim3,
  9. Mo Sun Kwon3,
  10. Bon Chul Koo3,
  11. Jeong Chan Ra4,
  12. Dae Yong Kim1,
  13. CheMyong Ko5,
  14. Byeong Chun Lee1,*

Article first published online: 8 APR 2009

DOI: 10.1002/dvg.20504

Issue

genesis

genesis

Volume 47, Issue 5, pages 314–322, May 2009

Additional Information

How to Cite

Hong, S. G., Kim, M. K., Jang, G., Oh, H. J., Park, J. E., Kang, J. T., Koo, O. J., Kim, T., Kwon, M. S., Koo, B. C., Ra, J. C., Kim, D. Y., Ko, C. and Lee, B. C. (2009), Generation of red fluorescent protein transgenic dogs. Genesis, 47: 314–322. doi: 10.1002/dvg.20504

Author Information

  1. 1

    College of Veterinary Medicine, Seoul National University, Seoul, Korea

  2. 2

    College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Korea

  3. 3

    Catholic University of Daegu School of Medicine, Daegu, Korea

  4. 4

    RNL Bio Co., Ltd., Seoul, Korea

  5. 5

    Department of Clinical Sciences, University of Kentucky, Lexington, Kentucky

*Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea

Publication History

  1. Issue published online: 20 MAY 2009
  2. Article first published online: 8 APR 2009
  3. Manuscript Accepted: 20 DEC 2008
  4. Manuscript Revised: 16 DEC 2008
  5. Manuscript Received: 8 OCT 2008

Funded by

  • KOSEF. Grant Number: M10625030005-08N250300510
  • BK21 Program for Veterinary Science
  • TS Corporation
  • RNL Bio

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Keywords:

  • dog;
  • transgenic;
  • fetal fibroblasts;
  • nuclear transfer;
  • red fluorescent protein

Abstract

Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene-expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP-fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP. genesis 47:314–322, 2009. © 2009 Wiley-Liss, Inc.

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