Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons
Article first published online: 23 APR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 47, Issue 6, pages 404–408, June 2009
How to Cite
Liang, Q., Kong, J., Stalker, J. and Bradley, A. (2009), Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons. Genesis, 47: 404–408. doi: 10.1002/dvg.20508
- Issue published online: 18 JUN 2009
- Article first published online: 23 APR 2009
- Manuscript Accepted: 13 JAN 2009
- Manuscript Revised: 12 JAN 2009
- Manuscript Received: 11 AUG 2008
- Wellcome Trust
- Sleeping Beauty;
- chromosomal mobilization efficiency;
- insertion site preference
The Sleeping Beauty and PiggyBac DNA transposon systems have recently been developed as tools for insertional mutagenesis. We have compared the chromosomal mobilization efficiency and insertion site preference of the two transposons mobilized from the same donor site in mouse embryonic stem (ES) cells under conditions in which there were no selective constraints on the transposons' insertion sites. Compared with Sleeping Beauty, PiggyBac exhibits higher transposition efficiencies, no evidence for local hopping and a significant bias toward reintegration in intragenic regions, which demonstrate its utility for insertional mutagenesis. Although Sleeping Beauty had no detectable genomic bias with respect to insertions in genes or intergenic regions, both Sleeping Beauty and PiggyBac transposons displayed preferential integration into actively transcribed loci. genesis 47:404–408, 2009. © 2009 Wiley-Liss, Inc.