Sox17-2A-iCre: A knock-in mouse line expressing Cre recombinase in endoderm and vascular endothelial cells
Version of Record online: 22 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 47, Issue 9, pages 603–610, September 2009
How to Cite
Engert, S., Liao, W. P., Burtscher, I. and Lickert, H. (2009), Sox17-2A-iCre: A knock-in mouse line expressing Cre recombinase in endoderm and vascular endothelial cells. Genesis, 47: 603–610. doi: 10.1002/dvg.20540
- Issue online: 24 SEP 2009
- Version of Record online: 22 JUN 2009
- Manuscript Accepted: 14 MAY 2009
- Manuscript Revised: 8 MAY 2009
- Manuscript Received: 2 MAR 2009
- Helmholtz Society
- Cre recombinase;
Sox17 encodes an SRY-related high-mobility group (HMG) box transcription factor that is essential for endoderm formation and fetal hematopoietic stem cell maintenance. In the mouse, expression of Sox17 is first observed in the extraembryonic endoderm and is subsequently seen in the definitive endoderm as well as in blood and the endothelial cells of the developing vasculature. To conditionally inactivate genes in these domains, we have targeted the Sox17 locus to generate a bicistronic mRNA linking Sox17 to a codon improved Cre recombinase (iCre) via a viral 2A sequence. Here we report a new Cre knock-in mouse line, Sox17-2A-iCre, with activity in the developing endoderm, the vascular endothelial cells of the cardiovascular system and the hematopoietic system. Our results indicate that the Sox17-2A-iCre is active in an early endoderm progenitor and recombination of the Rosa26 reporter was observed in all previously reported expression domains of Sox17. The Sox17-2A-iCre line will be an excellent tool to conditionally inactivate genes in the definitive endoderm as well as in the vasculature and hematopoietic system. genesis 47:603–610, 2009. © 2009 Wiley-Liss, Inc.