The last two authors contributed equally.
Ubiquitous expression of the monomeric red fluorescent protein mcherry in transgenic mice
Article first published online: 19 NOV 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 48, Issue 12, pages 723–729, December 2010
How to Cite
Fink, D., Wohrer, S., Pfeffer, M., Tombe, T., Ong, C. J. and Sorensen, P. H.B. (2010), Ubiquitous expression of the monomeric red fluorescent protein mcherry in transgenic mice. Genesis, 48: 723–729. doi: 10.1002/dvg.20677
- Issue published online: 16 DEC 2010
- Article first published online: 19 NOV 2010
- Accepted manuscript online: 17 SEP 2010 12:14PM EST
- Manuscript Accepted: 9 SEP 2010
- Manuscript Received: 7 OCT 2009
The use of the green fluorescent protein (GFP) to label specific cell types and track gene expression in animal models, such as mice, has evolved to become an essential tool in biological research. Transgenic animals expressing genes of interest linked to GFP, either as a fusion protein or transcribed from an internal ribosomal entry site (IRES) are widely used. Enhanced GFP (eGFP) is the most common form of GFP used for such applications. However, a red fluorescent protein (RFP) would be highly desirable for use in dual-labeling applications with GFP derived fluorescent proteins, and for deep in vivo imaging of tissues. Recently, a new generation of monomeric (m)RFPs, such as monomeric (m)Cherry, has been developed that are potentially useful experimentally. mCherry exhibits brighter fluorescence, matures more rapidly, has a higher tolerance for N-terminal fusion proteins, and is more photostable compared with its predecessor mRFP1. mRFP1 itself was the first true monomer derived from its ancestor DsRed, an obligate tetramer in vivo. Here, we report the successful generation of a transgenic mouse line expressing mCherry as a fluorescent marker, driven by the ubiquitin-C promoter. mCherry is expressed in almost all tissues analyzed including pre- and post-implantation stage embryos, and white blood cells. No expression was detected in erythrocytes and thrombocytes. Importantly, we did not encounter any changes in normal development, general physiology, or reproduction. mCherry is spectrally and genetically distinct from eGFP and, therefore, serves as an excellent red fluorescent marker alone or in combination with eGFP for labelling transgenic animals. genesis 48:723–729, 2010. © 2010 Wiley-Liss, Inc.