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FGF receptor gene expression and its regulation by FGF signaling during early zebrafish development

Authors

  • Satoshi Ota,

    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
    Current affiliation:
    1. Division of Cell Regulation Systems, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
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    • Satoshi Ota and Noriko Tonou-Fujimori contributed equally to this work.

  • Noriko Tonou-Fujimori,

    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
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    • Satoshi Ota and Noriko Tonou-Fujimori contributed equally to this work.

  • Noriko Tonou-Fujimori,

    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
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  • Yukiko Nakayama,

    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
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  • Yuki Ito,

    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
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  • Akinori Kawamura,

    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
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  • Kyo Yamasu

    Corresponding author
    1. Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
    2. Saitama University Brain Science Institute, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan
    • 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, Japan
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Abstract

The expression of all four fgfr genes was extensively examined throughout early embryogenesis of the zebrafish (Danio rerio). fgfr1 alone was expressed maternally throughout the blastoderm, and then zygotically in the anterior neural plate and presomitic mesoderm. fgfr4 expression was first detected in late blastulae and was gradually restricted to the brain. fgfr2 and fgfr3 expression were initiated in early and late gastrulae, respectively; fgfr2 was expressed in the anterior neural plate and somitic mesoderm, whereas fgfr3 was activated in the axial mesoderm and then in the midbrain and somitic mesoderm. During somitogenesis, each of these fgfr genes was expressed in a characteristic manner in the brain. Using an FGF signal inhibitor, dominant-negative FGF receptors and fgf8.1/fgf8a mutants, we found that fgfr expression is directly or indirectly regulated by FGF signaling during epiboly and at the end of somitogenesis, revealing the presence of an autoregulatory mechanism. genesis 48:707–716, 2010. © 2010 Wiley-Liss, Inc.

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