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Generation of two modified mouse alleles of the Hic1 tumor suppressor gene

  1. Vendula Pospichalova1,
  2. Jolana Tureckova1,
  3. Bohumil Fafilek1,
  4. Martina Vojtechova1,
  5. Michaela Krausova1,
  6. Jan Lukas1,
  7. Eva Sloncova1,
  8. Sylvia Takacova2,
  9. Vladimir Divoky2,
  10. Dominique Leprince3,
  11. Jiri Plachy1,
  12. Vladimir Korinek1,*

Article first published online: 22 MAR 2011

DOI: 10.1002/dvg.20719

Issue

genesis

genesis

Volume 49, Issue 3, pages 142–151, March 2011

Additional Information

How to Cite

Pospichalova, V., Tureckova, J., Fafilek, B., Vojtechova, M., Krausova, M., Lukas, J., Sloncova, E., Takacova, S., Divoky, V., Leprince, D., Plachy, J. and Korinek, V. (2011), Generation of two modified mouse alleles of the Hic1 tumor suppressor gene. Genesis, 49: 142–151. doi: 10.1002/dvg.20719

Author Information

  1. 1

    Department of Cell and Developmental Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic

  2. 2

    Department of Biology, Faculty of Medicine, Palacky University, 77515 Olomouc, Czech Republic

  3. 3

    CNRS UMR 8161, Institut de Biologie de Lille, Université Lille Nord de France, Institut Pasteur de Lille, IFR 142, 59017 Lille Cedex, France

*Institute of Molecular Genetics AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic

Publication History

  1. Issue published online: 22 MAR 2011
  2. Article first published online: 22 MAR 2011
  3. Accepted manuscript online: 20 JAN 2011 11:09AM EST
  4. Manuscript Accepted: 12 JAN 2011
  5. Manuscript Revised: 10 JAN 2011
  6. Manuscript Received: 3 DEC 2010

Funded by

  • Grant Agency of the Czech Republic. Grant Numbers: 204/07/1567, 204/09/H058
  • Grant Agency of Charles University in Prague. Grant Number: 43-251027
  • Academy of Sciences of the Czech Republic. Grant Number: AV0Z50520514

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Keywords:

  • hypermethylated in cancer 1;
  • Hic1 tumor suppressor;
  • gene targeting;
  • a conditional null allele;
  • citrine reporter mice;
  • placenta

Abstract

HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186–893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine “reporter” mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. genesis 49:142-151, 2011. © 2011 Wiley-Liss, Inc.

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