Technology Report

You have free access to this content

Generation of transgenic dogs that conditionally express green fluorescent protein

  1. Min Jung Kim1,
  2. Hyun Ju Oh1,
  3. Jung Eun Park1,
  4. Geon A. Kim1,
  5. So Gun Hong1,
  6. Goo Jang1,
  7. Mo Sun Kwon2,
  8. Bon Chul Koo2,
  9. Teoan Kim2,*,
  10. Sung Keun Kang3,
  11. Jeong Chan Ra3,
  12. CheMyong Ko4,
  13. Byeong Chun Lee1,*

Article first published online: 31 MAY 2011

DOI: 10.1002/dvg.20737

Issue

genesis

genesis

Volume 49, Issue 6, pages 472–478, June 2011

Additional Information

How to Cite

Kim, M. J., Oh, H. J., Park, J. E., Kim, G. A., Hong, S. G., Jang, G., Kwon, M. S., Koo, B. C., Kim, T., Kang, S. K., Ra, J. C., Ko, C. and Lee, B. C. (2011), Generation of transgenic dogs that conditionally express green fluorescent protein. Genesis, 49: 472–478. doi: 10.1002/dvg.20737

Author Information

  1. 1

    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 151-744, Korea

  2. 2

    Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea

  3. 3

    Stem Cell, RNL BIO Ltd., Seoul, 153-768, Korea

  4. 4

    Department of Clinical Sciences, University of Kentucky, Lexington, Kentucky, USA

*Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea

Publication History

  1. Issue published online: 10 JUN 2011
  2. Article first published online: 31 MAY 2011
  3. Accepted manuscript online: 22 FEB 2011 09:59AM EST
  4. Manuscript Accepted: 7 FEB 2011
  5. Manuscript Revised: 4 FEB 2011
  6. Manuscript Received: 11 AUG 2010

Funded by

  • MKE. Grant Number: 10033805-2010- 12, 10033839-2010-12
  • NRF. Grant Number: 20100025515, 20100002068
  • Research Institute for Veterinary Science and IPET. Grant Number: 109023-05-1-CG000
  • RNL BIO. Ltd.. Grant Number: 0468-20110001

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (496K)

Keywords:

  • dog;
  • transgenic;
  • somatic cell nuclear transfer;
  • tetracycline vector

Abstract

We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies. genesis 49:472–478, 2011. © 2011 Wiley-Liss, Inc.

View Full Article with Supporting Information (HTML) Get PDF (496K)