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Stella-Cre mice are highly efficient Cre deleters

  1. Hui Liu1,2,
  2. Wei Wang2,
  3. Su-Kit Chew2,
  4. Song-Choon Lee2,
  5. Juan Li3,
  6. George S. Vassiliou1,
  7. Tony Green3,
  8. P. Andrew Futreal2,
  9. Allan Bradley2,
  10. Shujun Zhang1,4,*,
  11. Pentao Liu2,4,*

Article first published online: 22 JUL 2011

DOI: 10.1002/dvg.20741

Issue

genesis

genesis

Volume 49, Issue 8, pages 689–695, August 2011

Additional Information

How to Cite

Liu, H., Wang, W., Chew, S.-K., Lee, S.-C., Li, J., Vassiliou, G. S., Green, T., Futreal, P. A., Bradley, A., Zhang, S. and Liu, P. (2011), Stella-Cre mice are highly efficient Cre deleters. Genesis, 49: 689–695. doi: 10.1002/dvg.20741

Author Information

  1. 1

    College of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China

  2. 2

    Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1HH, United Kingdom

  3. 3

    Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, United Kingdom

  4. 4

    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom

*College of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China 430070

Publication History

  1. Issue published online: 9 AUG 2011
  2. Article first published online: 22 JUL 2011
  3. Accepted manuscript online: 24 FEB 2011 03:13PM EST
  4. Manuscript Accepted: 10 FEB 2011
  5. Manuscript Received: 3 JAN 2010

Funded by

  • Wellcome Trust
  • China Scholarship Council

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Keywords:

  • Stella;
  • Cre-loxP recombination;
  • knock-in

Abstract

Cre-loxP recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella-Cre, where Cre expression cassette was targeted to the 3′ UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre-loxP recombination efficiency in Stella-Cre mice was investigated at several genomic loci including Rosa26, Jak2, and Npm1. At all the loci examined, we observed 100% Cre-loxP recombination efficiency in the embryos and in the germline. Thus, Stella-Cre mice serve as a very efficient deleter line. genesis 49:689–695, 2011. © 2011 Wiley-Liss, Inc.

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