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Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132

Authors

  • Tetsuo Ono,

    Corresponding author
    1. Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    2. Department of Medical Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan
    • Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
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  • Eiji Mizutani,

    1. Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    Current affiliation:
    1. Research Team for Reproductive Biology and Technology, National Institute of Livestock and Grassland Science, Tsukuba 305-0901, Japan
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  • Chong Li,

    1. Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    2. Department of Bioscience, Graduate School of Science and Technology, Kwansei Gakuin University, Sanda 662-8501, Japan
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  • Kazuo Yamagata,

    1. Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    Current affiliation:
    1. Center for genetic Analysis of Biological Responses, Research Institute for Microbial Disease, Osaka 565-0871, Japan
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  • Teruhiko Wakayama

    Corresponding author
    1. Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan
    2. Department of Medical Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan
    3. Department of Bioscience, Graduate School of Science and Technology, Kwansei Gakuin University, Sanda 662-8501, Japan
    • Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
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Abstract

Postovulatory mammalian oocytes age significantly in culture. B6D2F1 or ICR strain mouse oocytes were collected 16 h after hCG injection and then cultured for up to 40 h post hCG at 37°C under 5% CO2 in air. After intracytoplasmic sperm injection (ICSI), B6D2F1 and ICR oocytes lost full-term developmental potential by 30 h and 26 h after hCG administration, respectively. However, using supplementation with 10 mM caffeine or 1-5 μM of MG132, we could obtain live offspring from oocytes at 34 h (BDF1, 5%–21%) or 28 h (ICR, 5%–18%), whereas none were obtained from untreated aged oocytes. Caffeine maintained normal meiotic spindle morphology, whereas MG132 maintained maturation-promoting factor activity. These treatments did not affect the potential of fresh oocytes for fertilization and subsequent development. Thus, it should be safe to use these chemicals in routine in vitro fertilization and offspring could be generated by ICSI of aged fertilization failed oocytes. genesis 49:460–471, 2011. © 2011 Wiley-Liss, Inc.

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