A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging

Authors

  • Go Shioi,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
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  • Hiroshi Kiyonari,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
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  • Takaya Abe,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
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  • Kazuki Nakao,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
    2. Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine Faculty of Medicine, University of Tokyo, Tokyo 113-0033, Japan
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  • Toshihiko Fujimori,

    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
    2. Division of Embryology, National Institute for Basic Biology (NIBB), Okazaki 444-8787, Japan
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  • Chuan-Wei Jang,

    1. Program in Developmental Biology, Baylor College of Medicine, Houston, Texas
    2. Department of Genetics, University of Texas M.D. Anderson Cancer Center, Houston, Texas
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  • Cheng-Chiu Huang,

    1. Program in Developmental Biology, Baylor College of Medicine, Houston, Texas
    2. Department of Genetics, University of Texas M.D. Anderson Cancer Center, Houston, Texas
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  • Haruhiko Akiyama,

    1. Department of Orthopaedic Surgery, Kyoto University, Kyoto 606-8507, Japan
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  • Richard R. Behringer,

    1. Program in Developmental Biology, Baylor College of Medicine, Houston, Texas
    2. Department of Genetics, University of Texas M.D. Anderson Cancer Center, Houston, Texas
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  • Shinichi Aizawa

    Corresponding author
    1. Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
    • Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan
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Abstract

Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations. genesis 49:570–578, 2011. © 2011 Wiley-Liss, Inc.

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