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A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging

  1. Go Shioi1,
  2. Hiroshi Kiyonari1,
  3. Takaya Abe1,
  4. Kazuki Nakao1,2,
  5. Toshihiko Fujimori1,3,
  6. Chuan-Wei Jang4,5,
  7. Cheng-Chiu Huang4,5,
  8. Haruhiko Akiyama6,
  9. Richard R. Behringer4,5,
  10. Shinichi Aizawa1,*

Article first published online: 17 JUN 2011

DOI: 10.1002/dvg.20758

Issue

genesis

genesis

Special Issue: Special Issue on Live Imaging

Volume 49, Issue 7, pages 570–578, July 2011

Additional Information

How to Cite

Shioi, G., Kiyonari, H., Abe, T., Nakao, K., Fujimori, T., Jang, C.-W., Huang, C.-C., Akiyama, H., Behringer, R. R. and Aizawa, S. (2011), A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging. Genesis, 49: 570–578. doi: 10.1002/dvg.20758

Author Information

  1. 1

    Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan

  2. 2

    Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine Faculty of Medicine, University of Tokyo, Tokyo 113-0033, Japan

  3. 3

    Division of Embryology, National Institute for Basic Biology (NIBB), Okazaki 444-8787, Japan

  4. 4

    Program in Developmental Biology, Baylor College of Medicine, Houston, Texas

  5. 5

    Department of Genetics, University of Texas M.D. Anderson Cancer Center, Houston, Texas

  6. 6

    Department of Orthopaedic Surgery, Kyoto University, Kyoto 606-8507, Japan

*Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan

Publication History

  1. Issue published online: 7 JUL 2011
  2. Article first published online: 17 JUN 2011
  3. Accepted manuscript online: 18 APR 2011 12:04PM EST
  4. Manuscript Accepted: 11 APR 2011
  5. Manuscript Revised: 15 MAR 2011
  6. Manuscript Received: 17 OCT 2010

Funded by

  • CDB
  • National Institutes of Health. Grant Number: HD30284
  • Ben F. Love Endowment
  • Kleberg Foundation

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Keywords:

  • Rosa26 locus;
  • conditional cell labeling;
  • dual cell labeling;
  • nuclear labeling;
  • membrane labeling

Abstract

Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations. genesis 49:570–578, 2011. © 2011 Wiley-Liss, Inc.

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