Toward high-content/high-throughput imaging and analysis of embryonic morphogenesis

Authors

  • Thai V. Truong,

    1. California Institute of Technology, Division of Biology, Beckman Institute, 1200 East California Blvd, Pasadena, California 91125
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  • Willy Supatto

    Corresponding author
    1. California Institute of Technology, Division of Biology, Beckman Institute, 1200 East California Blvd, Pasadena, California 91125
    2. Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR 7645, INSERM U696, Palaiseau, France
    • Ecole Polytechnique, CNRS UMR 7645, INSERM U696, Palaiseau, France
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Abstract

In vivo study of embryonic morphogenesis tremendously benefits from recent advances in live microscopy and computational analyses. Quantitative and automated investigation of morphogenetic processes opens the field to high-content and high-throughput strategies. Following experimental workflow currently developed in cell biology, we identify the key challenges for applying such strategies in developmental biology. We review the recent progress in embryo preparation and manipulation, live imaging, data registration, image segmentation, feature computation, and data mining dedicated to the study of embryonic morphogenesis. We discuss a selection of pioneering studies that tackled the current methodological bottlenecks and illustrated the investigation of morphogenetic processes in vivo using quantitative and automated imaging and analysis of hundreds or thousands of cells simultaneously, paving the way for high-content/high-throughput strategies and systems analysis of embryonic morphogenesis. genesis 49:555–569, 2011. © 2011 Wiley-Liss, Inc.

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