Identification of novel micrornas in Xenopus laevis metaphase ii arrested eggs
Article first published online: 16 FEB 2012
Copyright © 2012 Wiley Periodicals, Inc.
Special Issue: Xenopus Special Issue
Volume 50, Issue 3, pages 286–299, March 2012
How to Cite
Ambady, S., Wu, Z. and Dominko, T. (2012), Identification of novel micrornas in Xenopus laevis metaphase ii arrested eggs. Genesis, 50: 286–299. doi: 10.1002/dvg.22010
- Issue published online: 2 MAR 2012
- Article first published online: 16 FEB 2012
- Accepted manuscript online: 5 JAN 2012 07:19AM EST
- Manuscript Accepted: 29 DEC 2011
- Manuscript Revised: 26 DEC 2011
- Manuscript Received: 13 SEP 2011
- NIH. Grant Number: R01GM085456
- deep sequencing;
Using a combination of deep sequencing and bioinformatics approach, we for the first time identify miRNAs and their relative abundance in mature, metaphase II arrested eggs in Xenopus laevis. We characterize 115 miRNAs that have been described either in Xenopus tropicalis (85), X. laevis (9), or other vertebrate species (21) that also map to known Xenopus pre-miRNAs and to the X. tropicalis genome. In addition, 72 new X. laevis putative candidate miRNAs are identified based on mapping to X. tropicalis genome within regions that have the propensity to form hairpin loops. These data expand on the availability of genetic information in X. laevis and identify target miRNAs for future functional studies. genesis 50:286–299, 2012. © 2012 Wiley Periodicals, Inc.