A novel transgenic line using the Cre–lox system to allow permanent lineage-labeling of the zebrafish neural crest

Authors

  • Frederico S.L.M. Rodrigues,

    1. Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath, United Kingdom
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  • Gail Doughton,

    1. Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath, United Kingdom
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  • Bingjie Yang,

    1. Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath, United Kingdom
    Current affiliation:
    1. Department of Research and Education, Shanghai Punan Hospital, Shanghai 200125, People's Republic of China
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  • Robert N. Kelsh

    Corresponding author
    1. Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath, United Kingdom
    • Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath BA2 7AY, United Kingdom
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Abstract

Accurate lineage tracing is crucial to understanding of developmental and stem cell biology, but is particularly challenging for transient and highly dispersive cell-types like the neural crest (NC). The authors report in this article a new zebrafish transgenic line Tg(-4725sox10:Cre)ba74. This line expresses Cre under the control of a well-characterized portion of the sox10 promoter and, by crossing to a floxed-reporter line, the authors show in this article that expression in this line is consistent with those described for GFP reporter lines using the same promoter. Reporter expression is readily detected in patterns consistent with the early expression domains. Thus, the authors see all major groups (pigment, neural, and skeletal) of NC-derived cell-types, as well as cell-types derived from the known non-NC sites of sox10 expression, including otic epithelium and oligodendrocytes. This line provides an invaluable tool for the further study of zebrafish NC development and NC-derived stem cells as well as that of the otic vesicle and oligodendrocytes. genesis 50:750–757, 2012. © 2012 Wiley Periodicals, Inc.

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