Defining progressive stages in the commitment process leading to embryonic lens formation
Article first published online: 19 JUN 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 50, Issue 10, pages 728–740, October 2012
How to Cite
Jin, H., Fisher, M. and Grainger, R. M. (2012), Defining progressive stages in the commitment process leading to embryonic lens formation. Genesis, 50: 728–740. doi: 10.1002/dvg.22038
- Issue published online: 19 OCT 2012
- Article first published online: 19 JUN 2012
- Accepted manuscript online: 8 MAY 2012 01:55AM EST
- Manuscript Accepted: 4 MAY 2012
- Manuscript Revised: 2 MAY 2012
- Manuscript Received: 20 APR 2012
- NIH. Grant Numbers: EY019000, EY017400
- Xenopus tropicalis
The commitment of regions of the embryo to form particular tissues or organs is a central concept in development, but the mechanisms controlling this process remain elusive. The well-studied model of lens induction is ideal for dissecting key phases of the commitment process. We find in Xenopus tropicalis, at the time of specification of the lens, i.e., when presumptive lens ectoderm (PLE) can be isolated, cultured, and will differentiate into a lens that the PLE is not yet irreversibly committed, or determined, to form a lens. When transplanted into the posterior of a host embryo lens development is prevented at this stage, while ∼ 3 h later, using the same assay, determination is complete. Interestingly, we find that specified lens ectoderm, when cultured, acquires the ability to become determined without further tissue interactions. Furthermore, we show that specified PLE has a different gene expression pattern than determined PLE, and that determined PLE can maintain expression of essential regulatory genes (e.g., foxe3, mafB) in an ectopic environment, while specified PLE cannot. These observations set the stage for a detailed mechanistic study of the genes and signals controlling tissue commitment. genesis 50:728–740, 2012. © 2012 Wiley Periodicals, Inc.