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A heat-inducible CRE/LOXP gene induction system in medaka

Authors

  • Kayo Kobayashi,

    1. Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki 444-8787, Japan
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  • Yasuhiro Kamei,

    1. Department for Applied Life Sciences, Spectrography and Bioimaging Facility, National Institute for Basic Biology, Okazaki 444-8585, Japan
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  • Masato Kinoshita,

    1. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
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  • Thomas Czerny,

    1. Department for Applied Life Sciences, University for Applied Sciences, FH Campus Wien, Helmuth Qualtinger Gasse 2, 1030 Vienna, Austria
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  • Minoru Tanaka

    Corresponding author
    1. Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki 444-8787, Japan
    2. Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi 444-8585, Japan
    • Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki 444-8787, Japan
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Abstract

We established three lines of transgenic medaka, a heat-shock element (HSE) monitor line (hse-GFP line), heat-inducible driver lines (hse-cre lines), and effector lines (gapdh-loxP[DsRed]-GFP lines). We employed these to comprehensively analyze gene induction at different time points in various tissues. These analyses demonstrate a good response of synthetic HSEs by heat treatment during embryogenesis and the mosaic gene induction by cre/loxP-mediated recombination, thus providing practical information regarding the feasibility of a heat-inducible cre/loxP-mediated system in medaka. We also activated recombination by local heat-treatment using a metal probe and an infrared laser. Our results collectively indicate that these lines allow us to perform lineage tracing and mosaic analysis and provide the platform to investigate gene functions at later developmental stage and adult. genesis 51:59–67, 2013. © 2012 Wiley Periodicals, Inc.

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