A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination
Article first published online: 25 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 51, Issue 4, pages 268–274, April 2013
How to Cite
Fukamachi, K., Tanaka, H., Sakai, Y., Alexander, D. B., Futakuchi, M., Tsuda, H. and Suzui, M. (2013), A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51: 268–274. doi: 10.1002/dvg.22371
- Issue published online: 18 APR 2013
- Article first published online: 25 FEB 2013
- Accepted manuscript online: 24 JAN 2013 12:04PM EST
- Manuscript Accepted: 14 JAN 2013
- Manuscript Revised: 26 NOV 2012
- Manuscript Received: 26 JUL 2012
- Japan Society for the Promotion of Science; the Ministry of Health, Labor and Welfare, Japan; the Ministry of Education, Culture, Sports, Science and Technology of Japan
The recent widespread application of Cre/loxP technology has resulted in a new generation of conditional animal models that can better recapitulate many salient features of human disease. These models benefit from the ability to monitor the expression and functionality of Cre protein. We have generated a conditional (Cre/loxP dependent) LacZ reporter rat (termed the LacZ541 rat) to monitor Cre in transgenic rats. When LacZ541 rats were bred with another transgenic rat line expressing Cre recombinase under the control of the CAG promoter, LacZ/Cre double transgenic embryos displayed ubiquitous expression of LacZ, and when LacZ541 rats were bred with transgenic rats expressing Cre/loxP-dependent oncogenic H- or K-ras, LacZ was expressed in the lesions resulting from the activation of the oncogene. The LacZ541 rat enables evaluation of the performance of Cre-expressing systems which are based upon transgenic rats or somatic gene transfer vectors and provides efficient and simple lineage marking. genesis 51:268–274. © 2013 Wiley Periodicals, Inc.