These authors contributed equally.
A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors
Article first published online: 21 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc.
How to Cite
Wiese, C. B., Fleming, N., Buehler, D. P. and Southard-Smith, E. M. (2013), A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors. Genesis. doi: 10.1002/dvg.22716
- Article first published online: 21 OCT 2013
- Accepted manuscript online: 3 OCT 2013 01:16PM EST
- Manuscript Accepted: 20 SEP 2013
- Manuscript Revised: 30 AUG 2013
- Manuscript Received: 25 FEB 2013
- NIH NIDDK . Grant Number: U01DK070219
- NIH NIDDK . Grant Number: RC1DK086594
- NIH NIDDK . Grant Number: R56 DK078158
- NIH . Grant Number: CA68485, DK20593, DK58404, HD15052, DK59637, EY08126
- Vanderbilt Ingram Cancer Center . Grant Number: P30 CA68485
- Vanderbilt Digestive Disease Research Center . Grant Number: P30DK058404
- US National Institutes of Health grants from NIDDK . Grant Number: R56DK078158, RC1DK086594, DK070219
- neural crest;
- neural progenitor;
- lower urinary tract;
- enteric nervous system;
- bacterial artificial chromosome (BAC);
Summary: Uchl1 encodes the protein gene product 9.5 antigen (PGP9.5) that is a widely used to identify migrating neural progenitors in the PNS, mature neurons of the central and peripheral nervous systems, as well as neuroendocrine cells. To facilitate analysis of developing peripheral neurons, we linked regulatory regions of Uchl1 carried within a 160kb bacterial artificial chromosome (BAC) to the dual fluorescent reporter H2BmCherry:GFP-gpi. The Uchl1-H2BmCherry:GFP-gpi transgene exhibits robust expression and allows clear discrimination of individual cells and cellular processes in cranial ganglia, sympathetic chain, the enteric nervous system (ENS), and autonomic ganglia of the urogenital system. The transgene also labels subsets of cells in endocrine tissues where earlier in situ hybridization (ISH) studies have previously identified expression of this deubiquinating enzyme. The Uchl1-H2BmCherry:GFP-gpi transgene will be a powerful tool for static and live imaging, as well as isolation of viable neural progenitors to investigate processes of autonomic neurogenesis. genesis 00:1–10. © 2013 Wiley Periodicals, Inc.