C. elegans nuclear receptor NHR-6 functionally interacts with the jun-1 transcription factor during spermatheca development

Authors

  • Chris R. Gissendanner,

    Corresponding author
    1. Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana
    • Correspondence to: Chris R. Gissendanner; Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana 71209. E-mail: gissendanner@ulm.edu

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  • Derrick Cardin,

    1. Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana
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  • Chris J. DuBose,

    1. Department of Biology, University of Louisiana at Monroe, Monroe, Louisiana
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  • Moustafa El Sayed,

    1. Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana
    2. Department of Biology, University of Louisiana at Monroe, Monroe, Louisiana
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  • Jeremy S. Harmson,

    1. Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana
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  • Brandon Praslicka,

    1. Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana
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  • Brian G. Rowan

    1. Department of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, Louisiana
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Summary

The NR4A nuclear receptor NHR-6 is an essential regulator of spermatheca organogenesis in C. elegans. In this study, we perform a focused, RNAi-based screen to identify modifiers of partial nhr-6 loss of function. Ninety-eight genes that encode signaling proteins expressed in the spermatheca were screened for enhancement of the nhr-6 RNAi phenotype. We identify the C. elegans gene jun-1, which encodes the homolog of the Jun transcription factor, as a strong enhancer of nhr-6 partial loss of function. We show that nhr-6 and jun-1 function together to regulate development of the spermatheca and are necessary for generating an organ with the normal number of cells. jun-1 is expressed in all cells of the developing spermatheca. We also provide evidence that NHR-6 and JUN-1 can physically interact in a yeast two-hybrid assay. Our results provide in vivo evidence that NR4A nuclear receptor and Jun transcription factor interactions are essential in regulating developmental processes in metazoans. genesis 52:29–38, 2014. © 2013 Wiley Periodicals, Inc.

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