Osama Odat and Jason Gardiner contributed equally to this study.
Characterization of an allelic series in the MONOPTEROS gene of arabidopsis
Article first published online: 5 DEC 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 52, Issue 2, pages 127–133, February 2014
How to Cite
Odat, O., Gardiner, J., Sawchuk, M. G., Verna, C., Donner, T. J. and Scarpella, E. (2014), Characterization of an allelic series in the MONOPTEROS gene of arabidopsis. Genesis, 52: 127–133. doi: 10.1002/dvg.22729
- Issue published online: 19 FEB 2014
- Article first published online: 5 DEC 2013
- Accepted manuscript online: 26 NOV 2013 10:55PM EST
- Manuscript Accepted: 22 NOV 2013
- Manuscript Revised: 20 NOV 2013
- Manuscript Received: 3 OCT 2013
- Discovery Grants of the Natural Sciences and Engineering Research Council of Canada (NSERC), NSERC CGS-M Scholarship, NSERC PGS-D Scholarship, NSERC CGS-M Scholarship, NSERC CGS-D Scholarship, NSERC CGS-M Scholarship, NSERC CGS-D Scholarship, Alberta Ingenuity Student Scholarships
- auxin signal transduction;
- cotyledon separation;
- root development;
- vein network patterning;
- vascular tissue differentiation
Patterning of numerous features of plants depends on transduction of the auxin signal. Auxin signaling is mediated by several pathways, the best understood of which relies on the function of the MONOPTEROS (MP) gene. Seven mp mutant alleles have been described in the widely used Columbia background of Arabidopsis: two extensively characterized and five only partially characterized. One of these five mp alleles appears to be extinct and thus unavailable for analysis. We show that two of the four remaining, partially characterized mp alleles reported to be in the Columbia background are in fact not in this background. We extend characterization of the remaining two Columbia alleles of mp, and we identify and characterize four new alleles of mp in the Columbia background, among which the first low-expression allele of mp and the strongest Columbia allele of mp. These genetic resources provide the research community with new experimental opportunities for insight into the function of MP-dependent auxin signaling in plant development. genesis 52:127–133. © 2013 Wiley Periodicals, Inc.