Epigenetic dysregulation of dopaminergic genes in eating disorders
Article first published online: 2 SEP 2009
Copyright © 2009 Wiley Periodicals, Inc.
International Journal of Eating Disorders
Volume 43, Issue 7, pages 577–583, 1 November 2010
How to Cite
Frieling, H., Römer, K. D., Scholz, S., Mittelbach, F., Wilhelm, J., De Zwaan, M., Jacoby, G. E., Kornhuber, J., Hillemacher, T. and Bleich, S. (2010), Epigenetic dysregulation of dopaminergic genes in eating disorders. Int. J. Eat. Disord., 43: 577–583. doi: 10.1002/eat.20745
- Issue published online: 2 SEP 2009
- Article first published online: 2 SEP 2009
- Manuscript Accepted: 3 JUL 2009
- eating disorders;
- anorexia nervosa;
- bulimia nervosa;
- dopamine transporter;
- dopamine receptors;
- gene expression;
- DNA methylation;
- real-time PCR
The pathophysiology of eating disorders such as anorexia nervosa (AN) and bulimia nervosa (BN) has been linked to an impaired dopaminergic neurotransmission, still the origin of this disturbance remains unknown. The aim of the present study was, therefore, to evaluate whether the expression of dopaminergic genes is altered in the blood of patients suffering from eating disorders and if these alterations can be explained by changes in the promoter specific DNA methylation of the genes.
We used quantitative real-time PCR to measure both the expression and the promoter specific DNA methylation of the dopamine transporter (DAT), and the D2 (DRD2) and D4 receptor (DRD4) gene in the blood of 46 patients (22 AN, 24 BN) and 30 healthy controls.
Patients showed an elevated expression of DAT mRNA when compared with the controls and a downregulation of the DRD2 expression. The upregulation of the DAT gene was accompanied by a hypermethylation of the gene's promoter in the AN and BN group while a significant hypermethylation of the DRD2 promoter was only present in the AN group. No differences in expression or methylation were found for the other dopamine receptors investigated.
Our study shows a disturbed expression of dopaminergic genes that is accompanied by a dysregulation of the epigenetic DNA methylation. Further studies are necessary to provide more insight into the epigenetic dysregulation of the dopaminergic neurotransmission in the pathophysiology of eating disorders. © 2009 by Wiley Periodicals, Inc. (Int J Eat Disord 2010;)