Supported by K01 DA024115 (to NCBM).
Identifying novel phenotypes of vulnerability and resistance to activity-based anorexia in adolescent female rats
Version of Record online: 13 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
International Journal of Eating Disorders
Volume 46, Issue 7, pages 737–746, November 2013
How to Cite
Barbarich-Marsteller, N. C., Underwood, M. D., Foltin, R. W., Myers, M. M., Walsh, B. T., Barrett, J. S. and Marsteller, D. A. (2013), Identifying novel phenotypes of vulnerability and resistance to activity-based anorexia in adolescent female rats. Int. J. Eat. Disord., 46: 737–746. doi: 10.1002/eat.22149
- Issue online: 28 OCT 2013
- Version of Record online: 13 JUL 2013
- Manuscript Revised: 29 APR 2013
- Manuscript Accepted: 29 APR 2013
- anorexia nervosa;
- activity-based anorexia;
- food restriction;
- animal model;
Activity-based anorexia is a translational rodent model that results in severe weight loss, hyperactivity, and voluntary self-starvation. The goal of our investigation was to identify vulnerable and resistant phenotypes of activity-based anorexia in adolescent female rats.
Sprague-Dawley rats were maintained under conditions of restricted access to food (N = 64; or unlimited access, N = 16) until experimental exit, predefined as a target weight loss of 30–35% or meeting predefined criteria for animal health. Nonlinear mixed effects statistical modeling was used to describe wheel running behavior, time to event analysis was used to assess experimental exit, and a regressive partitioning algorithm was used to classify phenotypes.
Objective criteria were identified for distinguishing novel phenotypes of activity-based anorexia, including a vulnerable phenotype that conferred maximal hyperactivity, minimal food intake, and the shortest time to experimental exit, and a resistant phenotype that conferred minimal activity and the longest time to experimental exit.
The identification of objective criteria for defining vulnerable and resistant phenotypes of activity-based anorexia in adolescent female rats provides an important framework for studying the neural mechanisms that promote vulnerability to or protection against the development of self-starvation and hyperactivity during adolescence. Ultimately, future studies using these novel phenotypes may provide important translational insights into the mechanisms that promote these maladaptive behaviors characteristic of anorexia nervosa. © 2013 Wiley Periodicals, Inc. (Int J Eat Disord 2013; 46:737–746)