This work was supported by Deutsche Forschungsgemeinschaft (grant VE247/3-1), by the Volkswagen Foundation, the South African National Biodiversity Institute, and EAZA.
Hiding deep in the trees: discovery of divergent mitochondrial lineages in Malagasy chameleons of the Calumma nasutum group
Article first published online: 11 JUN 2012
© 2011 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.
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Ecology and Evolution
Volume 2, Issue 7, pages 1468–1479, July 2012
How to Cite
Gehring, P.-S., Tolley, K. A., Eckhardt, F. S., Townsend, T. M., Ziegler, T., Ratsoavina, F., Glaw, F. and Vences, M. (2012), Hiding deep in the trees: discovery of divergent mitochondrial lineages in Malagasy chameleons of the Calumma nasutum group. Ecology and Evolution, 2: 1468–1479. doi: 10.1002/ece3.269
- Issue published online: 6 JUL 2012
- Article first published online: 11 JUN 2012
- Received: 17 January 2012; Revised: 28 March 2012; Accepted: 31 March 2012
- Calumma nasutum group;
- genital morphology;
- molecular diversity;
- snout appendage scales
We conducted a comprehensive molecular phylogenetic study for a group of chameleons from Madagascar (Chamaeleonidae: Calumma nasutum group, comprising seven nominal species) to examine the genetic and species diversity in this widespread genus. Based on DNA sequences of the mitochondrial gene (ND2) from 215 specimens, we reconstructed the phylogeny using a Bayesian approach. Our results show deep divergences among several unnamed mitochondrial lineages that are difficult to identify morphologically. We evaluated lineage diversification using a number of statistical phylogenetic methods (general mixed Yule-coalescent model; SpeciesIdentifier; net p-distances) to objectively delimit lineages that we here consider as operational taxonomic units (OTUs), and for which the taxonomic status remains largely unknown. In addition, we compared molecular and morphological differentiation in detail for one particularly diverse clade (the C. boettgeri complex) from northern Madagascar. To assess the species boundaries within this group we used an integrative taxonomic approach, combining evidence from two independent molecular markers (ND2 and CMOS), together with genital and other external morphological characters, and conclude that some of the newly discovered OTUs are separate species (confirmed candidate species, CCS), while others should best be considered as deep conspecific lineages (DCLs). Our analysis supports a total of 33 OTUs, of which seven correspond to described species, suggesting that the taxonomy of the C. nasutum group is in need of revision.