Hiding deep in the trees: discovery of divergent mitochondrial lineages in Malagasy chameleons of the Calumma nasutum group

Authors

  • Philip-Sebastian Gehring,

    1. Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany
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  • Krystal A. Tolley,

    1. Applied Biodiversity Research Division, South African National Biodiversity Institute, Claremont 7735, Cape Town, South Africa
    2. Department of Botany and Zoology, University of Stellenbosch, Matieland 7602, South Africa
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  • Falk Sebastian Eckhardt,

    1. Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany
    2. Tierärztliche Hochschule Hannover, Zoological Institute, Bünteweg 17, 30559 Hannover, Germany
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  • Ted M. Townsend,

    1. Department of Biology, San Diego State University, San Diego, California 92182
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  • Thomas Ziegler,

    1. Cologne Zoo, Riehler Straße 173, 50735 Köln, Germany
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  • Fanomezana Ratsoavina,

    1. Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany
    2. Département de Biologie Animale, Université d’Antananarivo, BP 906, Antananarivo 101, Madagascar
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  • Frank Glaw,

    1. Zoologische Staatssammlung München, Münchhausenstr. 21, 81247 München, Germany
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  • Miguel Vences

    1. Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany
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  • This work was supported by Deutsche Forschungsgemeinschaft (grant VE247/3-1), by the Volkswagen Foundation, the South African National Biodiversity Institute, and EAZA.

Miguel Vences, Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany. Tel: +49 531 391 3237; Fax: +49 531 391 8298; E-mail: m.vences@tu-bs.de

Abstract

We conducted a comprehensive molecular phylogenetic study for a group of chameleons from Madagascar (Chamaeleonidae: Calumma nasutum group, comprising seven nominal species) to examine the genetic and species diversity in this widespread genus. Based on DNA sequences of the mitochondrial gene (ND2) from 215 specimens, we reconstructed the phylogeny using a Bayesian approach. Our results show deep divergences among several unnamed mitochondrial lineages that are difficult to identify morphologically. We evaluated lineage diversification using a number of statistical phylogenetic methods (general mixed Yule-coalescent model; SpeciesIdentifier; net p-distances) to objectively delimit lineages that we here consider as operational taxonomic units (OTUs), and for which the taxonomic status remains largely unknown. In addition, we compared molecular and morphological differentiation in detail for one particularly diverse clade (the C. boettgeri complex) from northern Madagascar. To assess the species boundaries within this group we used an integrative taxonomic approach, combining evidence from two independent molecular markers (ND2 and CMOS), together with genital and other external morphological characters, and conclude that some of the newly discovered OTUs are separate species (confirmed candidate species, CCS), while others should best be considered as deep conspecific lineages (DCLs). Our analysis supports a total of 33 OTUs, of which seven correspond to described species, suggesting that the taxonomy of the C. nasutum group is in need of revision.

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