CSIRO Food Futures National Research Flagship.
Genetic analysis of Black Tiger shrimp (Penaeus monodon) across its natural distribution range reveals more recent colonization of Fiji and other South Pacific islands
Article first published online: 22 JUL 2012
© 2012 The Authors. Ecology and Evolution published by Blackwell Publishing Ltd.
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Ecology and Evolution
Volume 2, Issue 8, pages 2057–2071, August 2012
How to Cite
Waqairatu, S. S., Dierens, L., Cowley, J. A., Dixon, T. J., Johnson, K. N., Barnes, A. C. and Li, Y. (2012), Genetic analysis of Black Tiger shrimp (Penaeus monodon) across its natural distribution range reveals more recent colonization of Fiji and other South Pacific islands. Ecology and Evolution, 2: 2057–2071. doi: 10.1002/ece3.316
- Issue published online: 6 AUG 2012
- Article first published online: 22 JUL 2012
- Manuscript Accepted: 8 JUN 2012
- Manuscript Revised: 5 JUN 2012
- Manuscript Received: 24 AUG 2011
- CSIRO Food Futures National Research Flagship
- mitochondrial DNA;
- Penaeus monodon;
- South Pacific islands
The Black Tiger shrimp (Penaeus monodon) has a natural distribution range from East Africa to the South Pacific Islands. Although previous studies of Indo-Pacific P. monodon have found populations from the Indian Ocean and Australasia to differ genetically, their relatedness to South Pacific shrimp remains unknown. To address this, polymorphisms at eight shared microsatellite loci and haplotypes in a 418-bp mtDNA-CR (control region) sequence were examined across 682 P. monodon from locations spread widely across its natural range, including the South Pacific islands of Fiji, Palau, and Papua New Guinea (PNG). Observed microsatellite heterozygosities of 0.82–0.91, allele richness of 6.85–9.69, and significant mtDNA-CR haplotype variation indicated high levels of genetic diversity among the South Pacific shrimp. Analysis of microsatellite genotypes using a Bayesian STRUCTURE method segregated Indo-Pacific P. monodon into eight distinct clades, with Palau and PNG shrimp clustering among others from Southeast Asia and eastern Australia, respectively, and Fiji shrimp clustering as a distinct group. Phylogenetic analyses of mtDNA-CR haplotypes delineated shrimp into three groupings, with shrimp from Fiji again being distinct by sharing no haplotypes with other populations. Depending on regional location, the genetic structures and substructures identified from the genotyping and mtDNA-CR haplotype phylogeny could be explained by Metapopulation and/or Member–Vagrant type evolutionary processes. Neutrality tests of mutation-drift equilibrium and estimation of the time since population expansion supported a hypothesis that South Pacific P. monodon were colonized from Southeast Asia and eastern Australia during the Pleistocene period over 60,000 years ago when land bridges were more expansive and linked these regions more closely.