Funded by United States Department of Agriculture (USDA)-National Institute of Food and Agriculture (NIFA) to MJS.
Abnormally high digestive enzyme activity and gene expression explain the contemporary evolution of a Diabrotica biotype able to feed on soybeans
Version of Record online: 19 JUL 2012
© 2012 The Authors. Ecology and Evolution published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Ecology and Evolution
Volume 2, Issue 8, pages 2005–2017, August 2012
How to Cite
Curzi, M. J., Zavala, J. A., Spencer, J. L. and Seufferheld, M. J. (2012), Abnormally high digestive enzyme activity and gene expression explain the contemporary evolution of a Diabrotica biotype able to feed on soybeans. Ecology and Evolution, 2: 2005–2017. doi: 10.1002/ece3.331
M.J.C. and J.A.Z. contributed equally to this work.
- Issue online: 6 AUG 2012
- Version of Record online: 19 JUL 2012
- Manuscript Accepted: 19 JUN 2012
- Manuscript Revised: 12 JUN 2012
- National Institute of Food and Agriculture (NIFA). Grant Number: #2009-35505-06012
|ece3331-sup-0001-FigureS1.tif||image/tif||1046K||Figure S1. Collection sites of adult WCR populations. Areas where rotation-resistant behavioral phenotype has been reported are illustrated in gray on the map. Rotation-resistant populations (black circles) were collected in LaSalle (LaSalle County), Minonk (Woodford County), and Urbana (Champaign County), IL, whereas wild-type populations (open circles) were collected in Ames (Story County), IA, Concord (Dixon County), NE, and Higginsville (Lafayette County), MO.|
|ece3331-sup-0002-FigureS2.tif||image/tif||583K||Figure S2. Protease gene expression of D. virgifera virgifera. (A) Quantitative RT-PCR expression analysis of the five cathepsin L-like protease genes previously described in D.v. virgifera (Koiwa et al. 2000; Bown et al. 2004). (B) Quantitative RT-PCR expression analysis of the two cathepsin B-like protease genes of D.v. virgifera (Bown et al. 2004). Bracketed numbers indicate the number of PCR cycles required to obtain visible DNA bands within the exponential range of the amplification curve. The figures are a composite of gels for each clone and population and contain images spliced into place.|
|ece3331-sup-0003-TableS1.doc||Word document||1525K||Table S1. Name and GenBank accession number of WCR cathepsin-like protease transcripts and sequence of both forward and reverse primers used in the experiments.|
|ece3331-sup-0004-TableS2.doc||Word document||1525K||Table S2. Sequence of the WCR cathepsin-like cDNA fragments amplified for expression analysis.|
|ece3331-sup-0005-TableS3.doc||Word document||1525K||Table S3. Pairwise comparisons of relative gene expression least squares means between WCR populations for each gene and treatment.|
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