ece3331-sup-0001-FigureS1.tifimage/tif1046KFigure S1. Collection sites of adult WCR populations. Areas where rotation-resistant behavioral phenotype has been reported are illustrated in gray on the map. Rotation-resistant populations (black circles) were collected in LaSalle (LaSalle County), Minonk (Woodford County), and Urbana (Champaign County), IL, whereas wild-type populations (open circles) were collected in Ames (Story County), IA, Concord (Dixon County), NE, and Higginsville (Lafayette County), MO.
ece3331-sup-0002-FigureS2.tifimage/tif583KFigure S2. Protease gene expression of D. virgifera virgifera. (A) Quantitative RT-PCR expression analysis of the five cathepsin L-like protease genes previously described in D.v. virgifera (Koiwa et al. 2000; Bown et al. 2004). (B) Quantitative RT-PCR expression analysis of the two cathepsin B-like protease genes of D.v. virgifera (Bown et al. 2004). Bracketed numbers indicate the number of PCR cycles required to obtain visible DNA bands within the exponential range of the amplification curve. The figures are a composite of gels for each clone and population and contain images spliced into place.
ece3331-sup-0003-TableS1.docWord document1525KTable S1. Name and GenBank accession number of WCR cathepsin-like protease transcripts and sequence of both forward and reverse primers used in the experiments.
ece3331-sup-0004-TableS2.docWord document1525KTable S2. Sequence of the WCR cathepsin-like cDNA fragments amplified for expression analysis.
ece3331-sup-0005-TableS3.docWord document1525KTable S3. Pairwise comparisons of relative gene expression least squares means between WCR populations for each gene and treatment.

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