This study was supported by the Bill and Melinda Gates Foundation (Grant no. 51466) as part of the ‘Regional Cassava Virus Diseases Diagnostic Project’ coordinated by Dr. Joseph Ndunguru.
Genetic diversity and geographic distribution of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) genotypes associated with cassava in East Africa
Article first published online: 1 OCT 2012
© 2012 The Authors. Ecology and Evolution published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Ecology and Evolution
Volume 2, Issue 11, pages 2749–2762, November 2012
How to Cite
Ecology and Evolution 2012; 2(11): 2749–2762
- Issue published online: 8 NOV 2012
- Article first published online: 1 OCT 2012
- Manuscript Revised: 8 AUG 2012
- Manuscript Accepted: 8 AUG 2012
- Manuscript Received: 4 APR 2012
- Bill and Melinda Gates Foundation. Grant Number: 51466
- Cytochrome oxidase I (mtCOI);
- genetic diversity;
- geographic distribution;
The genetic variability of whitefly (Bemisia tabaci) species, the vectors of cassava mosaic begomoviruses (CMBs) in cassava growing areas of Kenya, Tanzania, and Uganda, was investigated through comparison of partial sequences of the mitochondria cytochrome oxidase I (mtCOI) DNA in 2010/11. Two distinct species were obtained including sub-Saharan Africa 1 (SSA1), comprising of two sub-clades (I and II), and a South West Indian Ocean Islands (SWIO) species. Among the SSA1, sub-clade I sequences shared a similarity of 97.8–99.7% with the published Uganda 1 genotypes, and diverged by 0.3–2.2%. A pairwise comparison of SSA1 sub-clade II sequences revealed a similarity of 97.2–99.5% with reference southern Africa genotypes, and diverged by 0.5–2.8%. The SSA1 sub-clade I whiteflies were widely distributed in East Africa (EA). In comparison, the SSA1 sub-clade II whiteflies were detected for the first time in the EA region, and occurred predominantly in the coast regions of Kenya, southern and coast Tanzania. They occurred in low abundance in the Lake Victoria Basin of Tanzania and were widespread in all four regions in Uganda. The SWIO species had a sequence similarity of 97.2–97.7% with the published Reunion sequence and diverged by 2.3–2.8%. The SWIO whiteflies occurred in coast Kenya only. The sub-Saharan Africa 2 whitefly species (Ug2) that was associated with the severe CMD pandemic in Uganda was not detected in our study.