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ece3480-sup-0001-FigureS1.epsimage/eps1545KFigure S1. Dialign-T alignment of inferred amino acid sequences for saratin/LAPP putative orthologs from Aliolimnatis fenestrata and Macrobdella decora together with the archetypal anticoagulants. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide regions, green boxes denote fully conserved cysteines, black boxes denote sites predicted to be under positive selection by FEL, and shading intensity corresponds to BLOSUM62 conservation. Afen, Aliolimnatis fenestrata; Hver, Hirudo verbana; Mdec, Macrobdella decora; Hoff, Haementeria officinalis; Hrob, Helobdella robusta; Hmed, Hirudo medicinalis. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0002-FigureS2.epsimage/eps269KFigure S2. Unrooted strict consensus of two equally parsimonious trees recovered from analysis of the bdellin data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0003-FigureS3.epsimage/eps240KFigure S3. Unrooted single most parsimonious tree recovered from analysis of the eglin c data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0004-FigureS4.epsimage/eps289KFigure S4. Unrooted single most parsimonious tree recovered from analysis of the antistasin-family data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0005-FigureS5.epsimage/eps1090KFigure S5. Dialign-T alignment of inferred amino acid sequences for antistasin-family putative orthologs from Aliolimnatis fenestrata, Hirudo verbana, and Macrobdella decora together with the archetypal anticoagulants. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide regions, green boxes denote fully conserved cysteines, black boxes denote sites predicted to be under positive selection by FEL, and yellow boxes denote sites predicted to be under positive selection by REL. Shading intensity corresponds to BLOSUM62 conservation. Afen, Aliolimnatis fenestrata; Hver, H. verbana; Mdec, Macrobdella decora; Hdep, Haementeria depressa; Hoff, Haementeria officinalis; Hghi, Haementeria ghilianii; Hnip, Hirudo nipponia; Hmed, Hirudo medicinalis; Ttes, Theromyzon tessulatum. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0006-FigureS6.epsimage/eps247KFigure S6. Unrooted single most parsimonious tree recovered from analysis of the hirudin data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0007-FigureS7.epsimage/eps632KFigure S7. Dialign-T alignment of inferred amino acid sequences for hirudin putative orthologs from Aliolimnatis fenestrata and Macrobdella decora together with the archetypal anticoagulants. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide region and green boxes denote fully conserved cysteines. Shading intensity corresponds to BLOSUM62 conservation. Afen, Aliolimnatis fenestrata; Mdec, Macrobdella decora; Hmed, Hirudo medicinalis; Hsyl, Hirudinaria sylvestris. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0008-FigureS8.epsimage/eps245KFigure S8. Unrooted single most parsimonious tree recovered from analysis of the manillase (heparanase-class endoglucuronidase) data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0009-FigureS9.epsimage/eps1104KFigure S9. Dialign-T alignment of inferred amino acid sequences for heparanase-class endoglucuronidase (manillase) putative orthologs from Aliolimnatis fenestrata, Hirudo verbana, and Macrobdella decora together with the archetypal anticoagulant. (a) The full alignment of orthologs across the known taxonomic diversity. Black boxes denote sites predicted to be under positive selection by FEL and yellow boxes denote sites predicted to be under positive selection by REL. Shading intensity corresponds to BLOSUM62 conservation. Afen, Aliolimnatis fenestrata; Hver, Hirudo verbana; Mdec, Macrobdella decora; Hmed, Hirudo medicinalis. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0010-FigureS10.epsimage/eps230KFigure S10. Unrooted single most parsimonious tree recovered from analysis of the decorsin data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0011-FigureS11.epsimage/eps415KFigure S11. Dialign-T alignment of inferred amino acid sequences for decorsin putative orthologs from Macrobdella decora together with the archetypal anticoagulants. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide region and green boxes denote fully conserved cysteines. Shading intensity corresponds to BLOSUM62 conservation. Mdec, Macrobdella decora. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0012-FigureS12.epsimage/eps253KFigure S12. Unrooted single most parsimonious tree recovered from analysis of the destabilase data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0013-FigureS13.epsimage/eps826KFigure S13. Dialign-T alignment of inferred amino acid sequences for destabilase putative orthologs from Macrobdella decora together with the archetypal anticoagulant. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide region, green boxes denote fully conserved cysteines, black boxes denote sites predicted to be under positive selection by FEL, and yellow boxes denote sites predicted to be under positive selection by REL. Shading intensity corresponds to BLOSUM62 conservation. Mdec, Macrobdella decora; Hmed, Hirudo medicinalis. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0014-FigureS14.epsimage/eps239KFigure S14. Unrooted single most parsimonious tree recovered from analysis of the ficolin data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0015-FigureS15.epsimage/eps777KFigure S15. Dialign-T alignment of inferred amino acid sequences for ficolin putative orthologs from Aliolimnatis fenestrata and Macrobdella decora together with the archetypal anticoagulant. (a) The full alignment of orthologs across the known taxonomic diversity. Green boxes denote fully conserved cysteines, black boxes denote sites predicted to be under positive selection by FEL, and shading intensity corresponds to BLOSUM62 conservation. Mdec, Macrobdella decora; Afen, Aliolimnatis fenestrata; Hmed, Hirudo medicinalis. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0016-FigureS16.epsimage/eps240KFigure S16. Unrooted single most parsimonious tree recovered from analysis of the leukocyte elastase inhibitor data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0017-FigureS17.epsimage/eps971KFigure S17. Dialign-T alignment of inferred amino acid sequences for leukocyte elastase inhibitor putative orthologs from Aliolimnatis fenestrata, Hirudo verbana, and Macrobdella decora together with the archetypal anticoagulant. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide region and shading intensity corresponds to BLOSUM62 conservation. Hver, Hirudo verbana; Afen, Aliolimnatis fenestrata; Mdec, Macrobdella decora; Hmed, Hirudo medicinalis. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.
ece3480-sup-0018-FigureS18.epsimage/eps245KFigure S18. Unrooted strict consensus of four equally parsimonious tree recovered from analysis of the c-type lectoxin data set (see also Table 3). When appropriate, GenBank accession numbers follow taxon names. Branch lengths are drawn proportional to change.
ece3480-sup-0019-FigureS19.epsimage/eps877KFigure S19. Dialign-T alignment of inferred amino acid sequences for c-type lectin putative orthologs from Aliolimnatis fenestrata, Hirudo verbana, and Macrobdella decora together with the archetypal anticoagulants. (a) The full alignment of orthologs across the known taxonomic diversity. Red boxes denote the predicted signal peptide region, green boxes denote fully or nearly fully conserved cysteines, and shading intensity corresponds to BLOSUM62 conservation. Hver, Hirudo verbana; Afen, Aliolimnatis fenestrata; Mdec, Macrobdella decora; Hmed, Hirudo medicinalis; Hoff, Haementeria officinalis. (b) Likelihood ratio test (LRT) scores for selection pressures at each site, plotted against codon position.

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