High-Throughput Sequencing: A Roadmap Toward Community Ecology

Authors

  • Timothée Poisot,

    Corresponding author
    1. Québec Centre for Biodiversity Sciences, Stewart Biological Sciences Building, Montréal, QC, Canada
    • Département de biologie, chimie et géographie, Université du Québec à Rimouski, Rimouski, QC, Canada
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    • All authors contributed equally.

  • Bérangère Péquin,

    1. Département de Biologie, Université Laval, Québec, QC, Canada
    2. Québec-Océan, Québec, QC, Canada
    3. Institut de Biologie Intégrative et des (IBIS) Systèmes, Québec, QC, Canada
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    • All authors contributed equally.

  • Dominique Gravel

    1. Département de biologie, chimie et géographie, Université du Québec à Rimouski, Rimouski, QC, Canada
    2. Québec Centre for Biodiversity Sciences, Stewart Biological Sciences Building, Montréal, QC, Canada
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    • All authors contributed equally.


Correspondence

Timothée Poisot, Département de biologie, chimie et géographie, Université du Québec à Rimouski, 300 Allée des Ursulines, Rimouski, QC, G5L 3A1, Canada. Tel: (1) 418 723-1986 - 1751; Fax: (1) 418 724-1849; E-mail: timothee.poisot@uqar.ca

Abstract

High-throughput sequencing is becoming increasingly important in microbial ecology, yet it is surprisingly under-used to generate or test biogeographic hypotheses. In this contribution, we highlight how adding these methods to the ecologist toolbox will allow the detection of new patterns, and will help our understanding of the structure and dynamics of diversity. Starting with a review of ecological questions that can be addressed, we move on to the technical and analytical issues that will benefit from an increased collaboration between different disciplines.

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