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ece3513-sup-0001-FigureS1.pdfapplication/PDF496KFigure S1. Results of GMYC test on an ultrametric tree produced using BEAST for the cytochrome oxidase I sequences. (A) Tree with ESUs and species names marked on the right-hand side. Support for nodes are posterior probabilities, shown only for species-level relationships and PP > 50%. Branches in red indicate population structure. (B) Maximum likelihood plot showing peak congruent with threshold limit in B. (C) Lineage through time plot with red line showing the threshold level suggested by GMYC.
ece3513-sup-0002-FigureS2.pdfapplication/PDF440KFigure S2. Results of GMYC test on an ultrametric tree produced using BEAST for concatenated sequences from all three mitochondrial genes. (A) Tree with ESUs and species names marked on the right-hand side. Support for nodes are posterior probabilities, shown only for species-level relationships and PP>50%. Branches in red indicate population structure. (B) Maximum likelihood plot showing peak congruent with threshold limit in B. (C) Lineage through time plot with red line showing the threshold level suggested by GMYC.
ece3513-sup-0003-FigureS3.pdfapplication/PDF346KFigure S3. Combined gene tree based on Bayesian inference using MrBayes for Solariellidae using four genes (28S, 16S, 12S and COI), showing only relationship among outgroups and Solariellidae (solariellid clade is collapsed). Support values are posterior probabilities (PP); branches with PP < 50% were collapsed, PP not shown for intraspecific relationships. See Table S1 for outgroup sampling details. Families and relationships are consistent with those discussed in Williams (2012).
ece3513-sup-0004-TableS1.docxWord document106KTable S1. Outgroup specimens used in study, ordered by family, along with details of sampling localities, registration numbers of voucher specimens, and EMBL accession numbers for sequences. MNHN, Muséum National d'Histoire Naturelle, Paris; NHMUK, Natural History Museum, London; and NSMT, National Museum of Nature and Science, Tokyo, Japan (NSMT). Photos of specimens are in Williams (2012) or available on MorphoBank online at http://www.morphobank.org/index.php/Projects/ProjectOverview/project_id/223.
ece3513-sup-0005-TableS2.docWord document44KTable S2. Forward (F) and reverse (R) PCR primers (also used in sequencing), and forward (FS) and reverse (RS) internal sequencing primers. Annealing temperatures and concentration of magnesium chloride (MgCl2) used in 50 μL polymerase chain reactions.

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