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FilenameFormatSizeDescription
ece3546-sup-0001-DataS1.fastatext/fas138KData S1. OTU sequences in FASTA format.
ece3546-sup-0002-DataS2.xlsxapplication/msexcel284KData S2. Summary of reads that passed quality filtering.
ece3546-sup-0003-DataS3.xlsxapplication/msexcel42KData S3. Comparison of molecular identification results for the 10 most common OTUs.
ece3546-sup-0004-DataS4.xlsxapplication/msexcel68KData S4. OTUs observed in root and soil samples (all OTUs).
ece3546-sup-0005-DataS5.xlsxapplication/msexcel163KData S5. Matrix representing the presence/absence of each fungal OTU in each root or soil sample.
ece3546-sup-0006-DataS6.xlsxapplication/msexcel45KData S6. Matrix representing the symbiosis of plant species and fungal OTUs.
ece3546-sup-0007-FigureS1.pdfapplication/PDF304KFigure S1. Map of the study plot. Schematic illustration of the sampling design in the study site. Root samples were collected on crossover points of the 1-m-mesh plot. Soil samples were collected on crossover points of the 2-m-mesh plot as indicated by the brown circles. Numbers indicate sample IDs in Data S2.
ece3546-sup-0008-FigureS2.pdfapplication/PDF5852KFigure S2. Example photographs of washed terminal roots. Example photographs of washed terminal roots. Each terminal root was washed in 70% ethanol by shaking it with 1-mm zirconium balls 15 times per second for 2 min using TissueLyser II (Qiagen).
ece3546-sup-0009-FigureS3.pdfapplication/PDF324KFigure S3. Example photographs of washed terminal roots. Molecular phylogeny of most commonly observed ascomycete OTUs. Maximum-likelihood topology based on ITS sequences is shown with bootstrap probabilities above the branches (>50%; 100 replicates). Fungal sequences of “dark septate endophytes” (Grünig et al. 2011; Newsham 2011) are indicated by asterisks. (A) OTUs in the order Helotiales (254 bp; J2ef + G model). (B) OTUs in the order Chaetothyriales (239 bp; TN93ef + G). (C) OTUs in the order Rhytismatales (258 bp; TN93ef + G).
ece3546-sup-0010-FigureS4.pdfapplication/PDF198KFigure S4. Molecular phylogeny of most commonly observed ascomycetes OTUs. Comparison of fungal community composition between root and soil samples. (A) Taxonomic composition of OTUs observed in root samples (98 of 172 OTUs identified at the order level). The analysis was conducted after converting the pyrosequencing data to the presence/absence matrix (see Materials and Methods; Data S5). Asterisks indicate OTUs whose order-level taxonomy is yet to be settled, but genus- or family-level taxonomic information is available. (B) Taxonomic information of OTUs observed in soil samples (41 of 90 OTUs identified at the order level). (C) Taxonomic information of OTUs observed in root samples (66 of 172 OTUs identified at the genus level). (D) Taxonomic information of OTUs observed in soil samples (30 of 90 OTUs identified at the genus level).

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