Community composition of root-associated fungi in a Quercus-dominated temperate forest: “codominance” of mycorrhizal and root-endophytic fungi
Article first published online: 5 APR 2013
© 2013 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Ecology and Evolution
Volume 3, Issue 5, pages 1281–1293, May 2013
How to Cite
Ecology and Evolution 2013; 3(5):1281–1293.
- Issue published online: 10 MAY 2013
- Article first published online: 5 APR 2013
- Manuscript Accepted: 9 MAR 2013
- Manuscript Received: 4 MAR 2013
- Japanese government. Grant Number: GS014
|ece3546-sup-0001-DataS1.fasta||text/fas||138K||Data S1. OTU sequences in FASTA format.|
|ece3546-sup-0002-DataS2.xlsx||application/msexcel||284K||Data S2. Summary of reads that passed quality filtering.|
|ece3546-sup-0003-DataS3.xlsx||application/msexcel||42K||Data S3. Comparison of molecular identification results for the 10 most common OTUs.|
|ece3546-sup-0004-DataS4.xlsx||application/msexcel||68K||Data S4. OTUs observed in root and soil samples (all OTUs).|
|ece3546-sup-0005-DataS5.xlsx||application/msexcel||163K||Data S5. Matrix representing the presence/absence of each fungal OTU in each root or soil sample.|
|ece3546-sup-0006-DataS6.xlsx||application/msexcel||45K||Data S6. Matrix representing the symbiosis of plant species and fungal OTUs.|
|ece3546-sup-0007-FigureS1.pdf||application/PDF||304K||Figure S1. Map of the study plot. Schematic illustration of the sampling design in the study site. Root samples were collected on crossover points of the 1-m-mesh plot. Soil samples were collected on crossover points of the 2-m-mesh plot as indicated by the brown circles. Numbers indicate sample IDs in Data S2.|
|ece3546-sup-0008-FigureS2.pdf||application/PDF||5852K||Figure S2. Example photographs of washed terminal roots. Example photographs of washed terminal roots. Each terminal root was washed in 70% ethanol by shaking it with 1-mm zirconium balls 15 times per second for 2 min using TissueLyser II (Qiagen).|
|ece3546-sup-0009-FigureS3.pdf||application/PDF||324K||Figure S3. Example photographs of washed terminal roots. Molecular phylogeny of most commonly observed ascomycete OTUs. Maximum-likelihood topology based on ITS sequences is shown with bootstrap probabilities above the branches (>50%; 100 replicates). Fungal sequences of “dark septate endophytes” (Grünig et al. 2011; Newsham 2011) are indicated by asterisks. (A) OTUs in the order Helotiales (254 bp; J2ef + G model). (B) OTUs in the order Chaetothyriales (239 bp; TN93ef + G). (C) OTUs in the order Rhytismatales (258 bp; TN93ef + G).|
|ece3546-sup-0010-FigureS4.pdf||application/PDF||198K||Figure S4. Molecular phylogeny of most commonly observed ascomycetes OTUs. Comparison of fungal community composition between root and soil samples. (A) Taxonomic composition of OTUs observed in root samples (98 of 172 OTUs identified at the order level). The analysis was conducted after converting the pyrosequencing data to the presence/absence matrix (see Materials and Methods; Data S5). Asterisks indicate OTUs whose order-level taxonomy is yet to be settled, but genus- or family-level taxonomic information is available. (B) Taxonomic information of OTUs observed in soil samples (41 of 90 OTUs identified at the order level). (C) Taxonomic information of OTUs observed in root samples (66 of 172 OTUs identified at the genus level). (D) Taxonomic information of OTUs observed in soil samples (30 of 90 OTUs identified at the genus level).|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.