Genetic structure of wildcat (Felis silvestris) populations in Italy
Article first published online: 22 JUN 2013
© 2013 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
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Ecology and Evolution
Volume 3, Issue 8, pages 2443–2458, August 2013
How to Cite
Ecology and Evolution 2013; 3(8): 2443–2458
- Issue published online: 12 AUG 2013
- Article first published online: 22 JUN 2013
- Manuscript Accepted: 19 MAR 2013
- Manuscript Revised: 15 MAR 2013
- Manuscript Received: 4 JAN 2013
- Italian Ministry of Environment
- University of California – Davis
- Center for Companion Animal Health
- Fundação para a Ciência e a Tecnologia. Grant Number: SFRH/BD/24361/2005
- Admixture analysis;
- African wildcat;
- conservation genetics;
- European wildcat;
- glacial refuges;
- landscape genetics
Severe climatic changes during the Pleistocene shaped the distributions of temperate-adapted species. These species survived glaciations in classical southern refuges with more temperate climates, as well as in western and eastern peripheral Alpine temperate areas. We hypothesized that the European wildcat (Felis silvestris silvestris) populations currently distributed in Italy differentiated in, and expanded from two distinct glacial refuges, located in the southern Apennines and at the periphery of the eastern Alps. This hypothesis was tested by genotyping 235 presumed European wildcats using a panel of 35 domestic cat-derived microsatellites. To provide support and controls for the analyses, 17 know wildcat x domestic cat hybrids and 17 Sardinian wildcats (F. s. libyca) were included. Results of Bayesian clustering and landscape genetic analyses showed that European wildcats in Italy are genetically subdivided into three well-defined clusters corresponding to populations sampled in: (1) the eastern Alps, (2) the peninsular Apennines, and (3) the island of Sicily. Furthermore, the peninsular cluster is split into two subpopulations distributed on the eastern (Apennine mountains and hills) and western (Maremma hills and lowlands) sides of the Apennine ridge. Simulations indicated Alpine, peninsular, and Sicilian wildcats were isolated during the Last Glacial Maximum. Population subdivision in the peninsula cluster of central Italy arose as consequence of a more recent expansions of historically or ecologically distinct European wildcat subpopulations associated with distinct the Continental or Mediterranean habitats. This study identifies previously unknown European wildcat conservation units and supports a deep phylogeographical history for Italian wildcats.