Selection on the Drosophila seminal fluid protein Acp62F
Article first published online: 23 MAY 2013
© 2013 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
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Ecology and Evolution
Volume 3, Issue 7, pages 1942–1950, July 2013
How to Cite
Ecology and Evolution 2013; 3(7): 1942–1950
- Issue published online: 10 JUL 2013
- Article first published online: 23 MAY 2013
- Manuscript Accepted: 25 APR 2013
- Manuscript Revised: 24 APR 2013
- Manuscript Received: 20 MAR 2013
- Natural Sciences and Engineering Research Council of Canada
- Experimental evolution;
- natural selection;
- protease inhibitor;
- sexual selection;
- sperm competition
Sperm competition and sexual conflict are thought to underlie the rapid evolution of reproductive proteins in many taxa. While comparative data are generally consistent with these hypotheses, few manipulative tests have been conducted and those that have provided contradictory results in some cases. Here, we use both comparative and experimental techniques to investigate the evolution of the Drosophila melanogaster seminal fluid protein Acp62F, a protease inhibitor for which extensive functional tests have yielded ambiguous results. Using between-species sequence comparisons, we show that Acp62F has been subject to recurrent positive selection. In addition, we experimentally evolved populations polymorphic for an Acp62F null allele over eight generations, manipulating the opportunities for natural and sexual selection. We found that the Acp62F null allele increased in frequency in the presence of natural selection, with no effect of sexual selection.