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ece3607-sup-0001-FigS1.tifimage/tif65KFigure S1. Mating effort and mating preference of focal males for the larger female in each of the repeated trials. Mating effort is depicted as the mean ± SE percent of total sexual acts directed by focal males toward the larger female, and mating preference as the mean ± SE percent time focal males spent associating with the larger female. The horizontal line denotes random choice between the paired stimulus females. The asterisk above individual histogram bars indicates a significant (< 0.0001; Wilcoxon signed-rank test) difference between the observed mean score and that expected from chance (i.e., 50%) for that particular trial. The P-value above each pair of histogram bars (paired trials 1 and 2) was obtained using linear mixed-effects models testing for any effect of trial number on the behavioral scores of males in each experiment.
ece3607-sup-0002-FigS2.tifimage/tif84KFigure S2. Male mating effort expressed as the mean ± SE frequency (number/40 min) of each of the different component sexual acts, and their total sum, directed by focal males toward each of the paired stimulus females over the course of the repeated paired trials. AP, approach; SD, courtship sigmoid display; GN, gonopodial nip; CA, copulation attempt. ***< 0.0001, obtained using the Wilcoxon signed-rank test.
ece3607-sup-0003-TableS1.tifimage/tif100KTable S1. Mean ± SE (range) standard body lengths of the focal males and paired stimulus females used on repeated consecutive days (paired trials 1 and 2), and the mean ± SE absolute and relative differences in the standard body length of the paired stimulus females used in individual behavioral trials. Individual focal males were tested repeatedly in paired trials 1 and 2.

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