ece3632-sup-0001-SupportingInformation.docxWord document426K

Figure S1. Factor loadings plotted against wavelength for PC1–PC4 that were used to describe variation in the reflectance spectra of male guppy orange spots. These data illustrate how the loadings of the principal components vary in relation to wavelength. PC1 is loaded positively across the full wavelength range and corresponds to orange spot brightness. PC2 is loaded positively in the wavelength range 475–575 nm and negatively in the range 400–450 nm and represents the relative amount of medium to short wavelength light reflected. PC3 is the amount of short-medium (425–525 nm) to medium-long (525–650 nm) wavelength light reflected while PC4 is weakly positively loaded below 425 nm and between 500–575 nm.

Figure S2. Principal component loadings for PC1–PC3 plotted against wavelength for male guppy iridescent spots. As for guppy orange spots, PC1 is positively loaded across all wavelengths and corresponds with spot brightness. PC2 represents the relative amount of short-medium (400–525 nm) to long (>550 nm) wavelength light reflected while PC3 corresponds to the amount of short (<450 nm) and long (>575 nm) wavelength light reflected relative to medium (450–575 nm) wavelength light reflected.

Figure S3. The thin-plate splines illustrating shape variation between high (red dots) and low (blue dots) quantity male guppy that were captured by the relative warp (RWs) scores. RW1 describes variation in the shape of the abdomen (flank), RW2 describes variation in the shape of the gill region and operculum plate, and RW3 describes the elongation of males’ caudal peduncle.

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