Testing potential selective agents acting on leaf shape in Ipomoea hederacea: predictions based on an adaptive leaf shape cline
Article first published online: 20 JUN 2013
© 2013 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
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Ecology and Evolution
Volume 3, Issue 8, pages 2409–2423, August 2013
How to Cite
Ecology and Evolution 2013; 3(8): 2409–2423
- Issue published online: 12 AUG 2013
- Article first published online: 20 JUN 2013
- Manuscript Accepted: 6 MAY 2013
- Manuscript Received: 29 APR 2013
- NSERC Canada
Table S1. Repeated measure ANOVAs of the effects of fixed variables (block, leaf shape, position of leaf, time, leaf shape × time, position of leaf × time) and random variables (family) on two ecophysiological traits (chlorophyll fluorescence and instantaneous temperature), measured on 11 clear and cold nights.
Table S2. Phenotypic correlation coefficients (Pearson) between all traits in the control treatment (above diagonal), and spray treatment (below the diagonal).
Table S3. Family means (genetic) correlation coefficients (Pearson) between all traits in the control treatment (above the diagonal), and the spray treatment (below the diagonal).
Table S4. Mixed model ANOVA statistics showing the effects of all fixed variables (block, treatment, type, leaf shape), and measured traits (damage, flowering time, RGRearly, RGRmid, RGRlate) on lifetime fitness (number of seeds) when only considering the two homozygote leaf shape classes (lobed and entire-shaped).
Table S5. Net fitness effects of leaf shape without controlling for the effects of other quantitative traits.
|ece3641-sup-0001-FigS1.pdf||application/PDF||109K||Figure S1. (A) The proportion of time that on average entire-shaped leaves (blue bars) or lobed leaves (green bars) are colder during the day. Each stacked bar represents a single day. (B) The difference in temperature (mean of entire leaves minus mean of lobed leaves ± SE; note, we only show SE toward zero) for each day. Points below the zero line indicate days that entires were on average colder, while points above zero indicate days that lobes were on average colder. In b, filled circles show nights where error bars do not cross zero, and blue solid lines represent the upper and lower maximum temperature differences.|
|ece3641-sup-0002-FigS2.pdf||application/PDF||97K||Figure S2. Mean (lsmeans ± 1 SE) chlorophyll fluorescence (Fv/Fm; circles and solid lines) and instantaneous temperature measurements (squares and dashed lines) for entire (blue), heterozygote (red), and lobed (green) leaf shape genotypes for 11 clear and cold nights in the autumn of 2010.|
|ece3641-sup-0003-FigS3.pdf||application/PDF||58K||Figure S3. Mean (lsmeans ± SE) seed production of individuals with each leaf shape after statistically removing the effects of insect damage, flowering time, all three growth rates, and our fixed effects described in the main text.|
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