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ece3650-sup-0001-FigS1.pdfapplication/PDF31KFigure S1. Schematic illustration of the Phoxinus phoxinus MHCIIB duplicates DAB 1 (A) and DAB 3 (B). Shaded boxes represent exons. The positions of the primers used in this study are indicated by arrows.
ece3650-sup-0002-Fig-S2.pdfapplication/PDF272KFigure S2. Details on sequence reads and quality scores from the 454 run. (A) Histogram showing sequence read quality score frequencies for all reads, bacteria 16S rDNA reads, DAB1 and DAB3 reads. Red line indicates the average quality score per class of reads (B) Histogram showing sequence read length frequencies for all reads, bacteria 16S rDNA reads, DAB1 and DAB3 reads. Red line indicates the average quality score per class of reads (C) Correlation between reads qualities and reads length for each class of reads. Red lines represent the least square regression between read quality and length. R statistics from correlation Pearson tests and their significance (*** significant at 99% level, ns nonsignificant at the 99% level) are indicated.
ece3650-sup-0003-TableS1.xlsapplication/msexcel44KTable S1. List of primers used to (A) characterize MHC Class II B in Phoxinus phoxinus; (B) amplify bacterial 16S rDNA and genotype MHC Class II B in P. phoxinus.
ece3650-sup-0004-TextS1.txtplain text document0KText S1. cDNA sequence amplified from Phoxinus phoxinus with FishEx12F-bis and FishC12S-R primers.
ece3650-sup-0005-TextS2.txtplain text document12KText S2. MHCIIB partial Exon 2 DAB1 and DAB3 sequences amplified using 454 sequencing.
ece3650-sup-0006-TextS3.txtplain text document154KText S3. 16s rDNA sequences amplified using universal bacteria primers and 454 sequencing.

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